Photo-immobilized heparin micropatterns on Ti-O surface: preparation, characterization, and evaluation in vitro
Photo-immobilized heparin micropatterns on Ti-O surface: preparation, characterization, and evaluation in vitro
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DOI：
10.1007/s10853-011-5634-1
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发表时间：
2011-11-01
影响因子：
4.5
通讯作者：
Huang, Nan
中科院分区：
文献类型：
--
作者：
Lei, Lijuan;Li, Chunhui;Huang, Nan
为了同时协调抗凝血和加速内皮化的功能，通过在3-氨丙基膦酸改性的氧化钛（Ti-O）基底上光固定肝素（用光反应部分官能化）来制造微图案。使用甲苯胺蓝测定法测定固定在表面上的肝素的量。利用扫描电子显微镜和表面轮廓仪对图案的表面形貌进行了分析。在体外研究血小板粘附和内皮细胞的粘附、增殖和定向行为。结果表明，与未图案化肝素样品相比，图案化肝素样品可降低血小板粘附，促进内皮细胞铺展和增殖。此外，适当尺寸的微条纹可以诱导细胞伸长并沿条纹方向沿着。这为血液接触装置的改进提供了一种新的方法。
In order to harmonize the functions of both anticoagulation and accelerating endothelialization simultaneously, the micropatterns were fabricated by photoimmobilizing heparin, functionalized with a photoreactive moiety, on 3-aminopropylphosphonic acid modified titanium oxide (Ti-O) substrates. The amount of heparin immobilized on the surfaces was determined using the toluidine blue assay. And the surface morphology of the patterns was examined using scanning electron microscopy and surface profiler. The platelet adhesion and endothelial cell behavior in terms of adhesion, proliferation, and orientation were investigated in vitro. It is clear that the heparin patterns can reduce the platelet adhesion, and promote endothelial cells spreading and proliferation compared to nonpatterned heparin sample. Furthermore, the microstripes with appropriate size can induce the cells to elongate and arrange along the stripe direction. This may suggest a new modification method for blood-contacting device.