Radioprotective effects of the thiols GSH and WR-2721 against X-ray-induction of micronuclei in erythroblasts
Radioprotective effects of the thiols GSH and WR-2721 against X-ray-induction of micronuclei in erythroblasts
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DOI：
10.1016/s1383-5718(00)00037-1
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发表时间：
2000-06-22
影响因子：
1.9
通讯作者：
Mazur, L
中科院分区：
文献类型：
--
作者：
Mazur, L
在接受还原型谷胱甘肽（CSII）和S-2-/3-氨丙基氨基/乙基硫代磷酸（WR-2721）（剂量为400 mg/kg体重）处理并暴露于6戈伊X射线的成年雄性Swiss小鼠的骨髓和外周血中评估微核嗜多染红细胞（MNPCE）的频率。GSH或WR-2721单独应用，或分别在X射线暴露前60和30分钟应用。在硫醇处理和X-照射后24小时测定MNPCE的数量。由GSH和WR-2721在小鼠成红细胞中引起的辐射保护和毒性，如MNPCE的数量所示，取决于所应用的硫醇。WR-2721给药后获得的辐射防护效果比GSH给药后更强。WR-2721的毒性大于GSH。在X射线暴露之前给予的GSN和WR-2721的组合与小鼠的相应单一药物治疗相比产生最大的辐射防护作用。两种硫醇的应用。与单独施用WR-2721或GSH相比，没有随后的X射线照射的施用似乎是最毒性的。GSH和WR-2721的联合作用对X射线诱导的小鼠成红细胞基因组不稳定性具有有效的辐射防护作用。(C)2000 Elsevier Science B. V.保留所有权利。
The frequency of micronucleated polychromatic erythrocytes (MNPCEs) was assessed in the bone marrow and peripheral blood of adult male Swiss mice treated with reduced glutathione (CSII) and S-2-/3-aminopropylamino/ethyl phosphorothioic acid (WR-2721), at a dose of 400 mg/kg body weight, and exposed to 6 Gy X-rays. GSH or WR-2721 was applied alone, or 60 and 30 min, respectively, prior to X-ray-exposure. The number of MNPCEs was determined at 24 h after the thiol treatment and X-irradiation. The radioprotection and toxicity caused in the mouse erythroblasts by GSH and WR-2721, as indicated by the number of MNPCEs were dependent on the thiol applied. The stronger radioprotective effect is obtained following WR-2721 administration than after GSH application. WR-2721 showed greater toxicity than GSH. The combination of GSN and WR-2721 given before X-ray-exposure resulted in the most radioprotective effect as compared to the respective single-drug treatment of mice. Application of the both thiols. without subsequent X-irradiation appeared to be the most toxic, compared with administration of WR-2721 or GSH alone. The effective radioprotection by the combined action of GSH and WR-2721 against genomic instability induced in the mouse erythroblasts by X-rays was shown. (C) 2000 Elsevier Science B.V. All rights reserved.