High resolution synchrotron imaging of wheat root hairs growing in soil and image based modelling of phosphate uptake
High resolution synchrotron imaging of wheat root hairs growing in soil and image based modelling of phosphate uptake
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DOI：
10.1111/nph.12294
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发表时间：
2013-06-01
期刊：
影响因子：
9.4
通讯作者：
Roose, Tiina
中科院分区：
文献类型：
--
作者：
Keyes, Samuel D.;Daly, Keith R.;Roose, Tiina
根毛对于微溶养分的吸收非常重要，特别是在养分缺乏的土壤中。日益复杂的数学模型的发展使得吸收特性得以量化。然而，建模一直受到限制，缺乏成像方法活根毛生长在真实的土壤。我们开发了一个植物生长协议，并使用同步辐射X射线层析显微镜（SRXTM），以揭示三维（3D）的根毛在真实的土壤中的相互作用。我们开发了一个模型的磷酸盐吸收的根毛直接根据几何形状的头发和相关的土壤孔隙所揭示的成像。以前的模拟研究发现，根毛主导磷酸盐的吸收。相比之下，我们的研究表明，头发和根的贡献是相等的。我们发现，头发的吸收是更本地化的比根，并强烈依赖于根毛和聚集体的方向。图像毛土相互作用的能力，使一个步骤的变化，建模方法，允许一个更现实的处理过程中的规模个别根毛在土壤孔隙。
Root hairs are known to be highly important for uptake of sparingly soluble nutrients, particularly in nutrient deficient soils. Development of increasingly sophisticated mathematical models has allowed uptake characteristics to be quantified. However, modelling has been constrained by a lack of methods for imaging live root hairs growing in real soils. We developed a plant growth protocol and used Synchrotron Radiation X-ray Tomographic Microscopy (SRXTM) to uncover the three-dimensional (3D) interactions of root hairs in real soil. We developed a model of phosphate uptake by root hairs based directly on the geometry of hairs and associated soil pores as revealed by imaging. Previous modelling studies found that root hairs dominate phosphate uptake. By contrast, our study suggests that hairs and roots contribute equally. We show that uptake by hairs is more localized than by roots and strongly dependent on root hair and aggregate orientation. The ability to image hairsoil interactions enables a step change in modelling approaches, allowing a more realistic treatment of processes at the scale of individual root hairs in soil pores.