Effects of elevated CO2 on activities of protective and detoxifying enzymes in Frankliniella occidentalis and F. intonsa under spinetoram stress
Effects of elevated CO2 on activities of protective and detoxifying enzymes in Frankliniella occidentalis and F. intonsa under spinetoram stress
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DOI：
10.1002/ps.6630
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发表时间：
2021-09-19
影响因子：
4.1
通讯作者：
Gui, Furong
中科院分区：
文献类型：
--
作者：
Fan, Zongfang;Qian, Lei;Gui, Furong
背景二氧化碳浓度升高会直接影响杀虫剂对昆虫的毒性和昆虫对杀虫剂的生理反应。西氏富兰氏菌(Frankliniella Ocicientis)和金斑潜蝇(F.intonsa)是以园艺作物为目标的极具破坏性的害虫。司匹托仑是一种防治蓟马的有效杀虫剂。本研究旨在探讨CO_2浓度升高对多菌灵对西花镰刀菌和叶枯病菌药效的影响，以及其对CO_2浓度升高下保护酶和解毒酶活性的影响。值得注意的是，可以在进一步的研究中利用这些酶来开发对蓟马抗药性管理的干预措施。结果毒力测定结果表明，在高浓度CO2(800亩L L~(-1)浓度)下对西花菜夜蛾和美洲斑潜蝇作用48h的LC_(50)值分别为0.08和0.006 mg L~(-1)，分别是环境CO_2(400亩L~(-1)浓度)下的0.62和0.75倍。结果表明，CO_2浓度升高降低了蓟马体内超氧化物歧化酶和乙酰胆碱酯酶的活性，而提高了羧酸酯酶和谷胱甘肽S转移酶的活性。然而，在两种CO2水平下，菠托拉姆提高了两种蓟马的保护酶和解毒酶的活性。CO_2浓度升高和刺激素对蓟马生理活性的影响存在协同作用，西洋蓟马的酶活性普遍高于台湾蓟马。结论CO_2浓度升高可增强多菌灵对蓟马的药效，西花菜对多菌胺的敏感性高于西药材，西药材比西药材对环境的适应性更强。
BACKGROUND Elevated CO2 can directly affect the toxicity of insecticides to insects and the physiological response of insects to insecticides. Frankliniella occidentalis and F. intonsa are highly destructive pests that target horticultural crops. Spinetoram is an effective pesticide against thrips. This study sought to explore the effect of elevated CO2 on efficacy of spinetoram against F. occidentalis and F. intonsa and effect of the spinetoram on activities of protective and detoxifying enzymes under elevated CO2. Notably, these enzymes can be exploited in further studies to develop interventions for thrips resistance management. RESULTS Toxicity bioassay showed that the LC50 values of F. occidentalis and F. intonsa exposed to spinetoram at elevated CO2 (800 mu L L-1 concentration) for 48 h was 0.08 and 0.006 mg L-1, respectively, which is 0.62 and 0.75 times of the values at ambient CO2 (400 mu L L-1 concentration). The findings showed that elevated CO2 decreased activities of the superoxide dismutase and acetylcholinesterase in thrips, while increasing the activities of carboxylesterase and glutathione S-transferase. However, spinetoram increased activities of protective and detoxifying enzymes in both thrips under the two CO2 levels. Elevated CO2 and spinetoram affect the physiological enzyme activity in thrips synergistically, and the activities of analyzed enzymes were generally higher in F. occidentalis than in F. intonsa. CONCLUSION Elevated CO2 amplifies the efficacy of spinetoram on thrips, F. intonsa is more susceptibility to spinetoram than F. occidentalis and the latter showed better adaptation to adverse conditions than the former.