Enhancement of radiation cytotoxicity in breast-cancer cells by localized attachment of gold nanoparticles
Enhancement of radiation cytotoxicity in breast-cancer cells by localized attachment of gold nanoparticles
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DOI：
10.1002/smll.200700794
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发表时间：
2008-09-01
期刊：
影响因子：
13.3
通讯作者：
Xing, James Z.
中科院分区：
文献类型：
--
作者：
Kong, Tao;Zeng, Jie;Xing, James Z.
合成了金纳米粒子（GNP）和修饰了半胱胺（AET）和硫代葡萄糖（Glu）两种功能分子的GNP。研究了乳腺癌细胞系（MCF-7）与非恶性乳腺细胞系（MCF-10A）的细胞摄取和辐射细胞毒性增强。透射电子显微镜（TEM）结果显示，癌细胞吸收功能性Glu-GNP显着超过裸GNP。TEM结果还表明，AET-加帽的GNP主要结合在MCF-7细胞膜上，而Glu-GNP进入细胞并分布在细胞质中。在MCF-7细胞摄取Glu-GNP或结合AET-GNP后，在24、48和72小时观察到体外细胞毒性效应。结果表明，这些功能性GNP对这些细胞具有很小的毒性或没有毒性。为了验证对癌细胞的增强的杀伤作用，将各种形式的辐射（例如200 kVp X射线和γ射线）施加到具有和不具有功能性GNP的细胞。通过与单独照射相比，结果表明GNP显著增强癌症杀伤。
Gold nanoparticles (GNPs) and modified GNPs having two kinds of functional molecules, cysteamine (AET) and thioglucose (Glu), are synthesized. Cell uptake and radiation cytotoxicity enhancement in a breast-cancer cell line (MCF-7) versus a nonmalignant breast-cell line (MCF-10A) are studied. Transmission electron microscopy (TEM) results show that cancer cells take up functional Glu-GNPs significantly more than naked GNPs. The TEM results also indicate that AET-capped GNPs are mostly bound to the MCF-7 cell membrane, while Glu-GNPs enter the cells and are distributed in the cytoplasm. After MCF-7 cell uptake of Glu-GNPs, or binding of AET-GNPs, the in vitro cytotoxicity effects are observed at 24, 48, and 72 hours. The results show that these functional GNPs have little or no toxicity to these cells. To validate the enhanced killing effect on cancer cells, various forms of radiation are applied such as 200 kVp X-rays and gamma-rays, to the cells, both with and without functional GNPs. By comparison with irradiation alone, the results show that GNPs significantly enhance cancer killing.