Effect of oestradiol on cytokine production in immortalized human marrow stromal cell lines.
Effect of oestradiol on cytokine production in immortalized human marrow stromal cell lines.
复制标题
雌二醇对永生化人骨髓基质细胞系细胞因子产生的影响。
DOI：
10.1006/cyto.2001.0956
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发表时间：
2001
期刊：
影响因子：
3.8
通讯作者：
M. Cohen
中科院分区：
文献类型：
--
作者：
A. C. Ramalho;A. Jullienne;P. Couttet;A. Graulet;C. Morieux;M. de Vernejoul;M. Cohen
雌激素缺乏促进骨破骨细胞生成和骨吸收。小鼠基质细胞和破骨细胞前体之间合作的证据表明，雌二醇通过调节基质细胞释放细胞因子发挥作用。骨髓基质含有多能祖细胞，其产生许多间充质谱系，包括可调节破骨细胞分化的成骨细胞。我们永生化并表征了6个人骨髓基质细胞系（Stro 1的存在，碱性磷酸酶，骨钙素的分泌，脂滴的形成，以及α和β雌激素受体的存在）。然后在体外评价细胞因子对雌二醇的反应，以及佛波醇肉豆蔻酸酯乙酸酯（PMA）刺激的细胞因子水平。细胞具有未分化基质细胞的特征（Stro 1+，RANK-L+），并表达α-雌激素受体。成骨细胞表型（碱性磷酸酶和骨钙素的量）较弱，分化为脂肪细胞的能力较差。这些细胞系不响应雌二醇产生白细胞介素6（IL-6），IL-1或肿瘤坏死因子α（TNF-α）无论是组成性或刺激后与PMA。此外，RANK-L和护骨素的表达在体外不受雌二醇的调节。因此，基质细胞对这些细胞因子的调节似乎不是雌二醇调节人体骨吸收的机制。
Oestrogen deficiency enhances bone osteoclastogenesis and bone resorption. Evidence of cooperation between stromal cells and osteoclast precursors in mice suggests that oestradiol acts by regulating cytokine release from stromal cells. Bone marrow stroma contains multipotent progenitors that give rise to many mesenchymal lineages, including osteoblasts that may regulate osteoclast differentiation. We immortalized and characterized six human bone marrow stromal cell lines (presence of Stro1, secretion of alkaline phosphatase, osteocalcin, formation of lipid droplets, and presence of alpha and beta oestrogen receptors). The response of cytokines to oestradiol was then evaluated in vitro, as were the phorbol myristate acetate (PMA)-stimulated cytokine levels. Cells had the characteristics of undifferentiated stromal cells (Stro1+, RANK-L+), and expressed alpha-oestrogen receptors. The osteoblast phenotype (amounts of alkaline phosphatase and osteocalcin) was weak and there was a poor capacity to differentiate into adipocytes. These cell lines did not respond to oestradiol by producing interleukin 6 (IL-6), IL-1 or tumour necrosis factor alpha (TNF-alpha) either constitutively or after stimulation with PMA. Moreover, RANK-L and osteoprotegerin expressions were not regulated by oestradiol in vitro. Thus, modulation of these cytokines by stromal cells do not appear to be the mechanism by which oestradiol regulates bone resorption in humans.
雌激素停药后，小鼠骨髓和骨细胞产生的白细胞介素 6 增加。
影响因子：
4.8
作者：
Passeri,G;Girasole,G;Jilka,RL;Manolagas,SC
通讯作者：
Manolagas,SC
影响因子：
4.8
作者：
L. Hofbauer;S. Khosla;C. Dunstan;D. Lacey;T. Spelsberg;B. Riggs
通讯作者：
L. Hofbauer;S. Khosla;C. Dunstan;D. Lacey;T. Spelsberg;B. Riggs