INDUCED DEVELOPMENT OF PROXIMAL TUBULAR NA-K-ATPASE, BASOLATERAL CELL-MEMBRANES AND FLUID REABSORPTION
INDUCED DEVELOPMENT OF PROXIMAL TUBULAR NA-K-ATPASE, BASOLATERAL CELL-MEMBRANES AND FLUID REABSORPTION
复制标题
DOI：
10.1111/j.1748-1716.1984.tb07439.x
复制
发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
LARSSON, L
中科院分区：
文献类型：
--
作者：
APERIA, A;LARSSON, L
肾上腺皮质激素（ACH）诱导大鼠未成熟近端小管（PT）细胞中na - atp酶活性。用裂滴法测定倍他米松（β）和醛固酮（aldo）对20日龄（幼龄）和40日龄（成年）大鼠PT基底膜和外侧细胞膜（BLM）大小、PT液体重吸收量（J v (a)）和PT nak - atp酶活性的影响。幼龄大鼠和成年大鼠血清ACH水平相同，但肾上腺切除术导致幼龄大鼠nak - atp酶活性下降幅度明显大于成年大鼠。土地面积(.mu.m2/.mu。m-3细胞体积)、jv (a)和NaK-ATPase均显著低于成年大鼠。3天的高剂量治疗（60 .mu）。G /100 G)或aldo (40 .mu。g/100 g)显著增加幼龄大鼠BLM表面积、jv (a)和NaKATPase活性，而成年大鼠无显著增加。肾上腺切除术后3 d，成年大鼠jv (a)明显下降。ach依赖性的NaKATPase活性变化与jv之间存在显著相关性(a)。短期高剂量乙酰胆碱治疗（2-3 h）未显著增加幼鼠NaKATPase活性和jv (a)。
Adrenal corticoid hormones (ACH) induce NaK-ATPase activity in immature proximal tubular (PT) cells in rats. The effect of betamethasone (beta) and aldosterone (aldo) on the size of PT basal and lateral cell membranes (BLM), PT fluid reabsorption (J v (a)) measured in vivo with the split drop technique and PT NaK-ATPase activity was determined in 20-day-old (young) and 40-day-old (adult) rats. Serum levels of ACH were the same in young and in adult rats, but adrenalectomy caused a significantly larger fall in NaK-ATPase activity in young than in adult rats. BLM surface area (.mu.m2/.mu.m-3 cell volume), J v (a) and NaK-ATPase were significantly lower in young than in adult rats. Three-day treatment with high doses of beta (60 .mu.g/100 g) or aldo (40 .mu.g/100 g) significantly increased BLM surface area, J v (a) and NaKATPase activity in young but not in adult rats. Three days after adrenalectomy, J v (a) was significantly depressed in adult rats. There was a significant correlation between the ACH-dependent changes in NaKATPase activity and J v (a). Short-term treatment (2-3 h) with high doses of ACH did not significantly increase NaKATPase activity and J v (a) in young rats.