IMMUNOHISTOCHEMICAL CHARACTERIZATION OF PLACENTAL NITRIC-OXIDE SYNTHASE EXPRESSION IN PREECLAMPSIA
IMMUNOHISTOCHEMICAL CHARACTERIZATION OF PLACENTAL NITRIC-OXIDE SYNTHASE EXPRESSION IN PREECLAMPSIA
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DOI：
10.1016/0002-9378(95)90324-0
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发表时间：
1995-09-01
影响因子：
9.8
通讯作者：
MYATT, L
中科院分区：
文献类型：
--
作者：
GHABOUR, MS;EIS, ALW;MYATT, L
目的：我们的目的是比较先兆子痫胎盘胎盘和脐带中内皮一氧化氮合酶的表达与血压正常胎盘的表达。 研究设计：我们比较先兆子痫（n = 3）与正常（n = 3）妊娠中胎盘内皮一氧化氮合酶的表达。用单克隆内皮一氧化氮合酶抗体（H32）对脐带、绒毛膜板血管和终末绒毛的冰冻切片进行免疫染色。结果：子痫前期和血压正常妊娠的脐带动静脉内皮、绒毛膜板血管和干绒毛血管的内皮一氧化氮合酶免疫染色没有差异。相反，在先兆子痫胎盘中，在具有底层平滑肌层的小末端绒毛血管中观察到内皮一氧化氮合酶免疫染色。在合体滋养层中，内皮一氧化氮合酶免疫染色在先兆子痫胎盘中主要出现在顶端，分布广泛，但在血压正常的胎盘中主要出现在基底和点状。结论：终末绒毛血管和合体滋养层中内皮一氧化氮合酶表达的差异可能是胎盘中发生血管改变或损伤的结果。先兆子痫。这些差异可能会改变先兆子痫中胎儿和母体胎盘脉管系统的血流调节。
OBJECTIVE: Our purpose was to compare the expression of endothelial nitric oxide synthase in the placenta and umbilical cord of preeclamptic placenta with that of the normotensive placenta.STUDY DESIGN: We compared placental endothelial nitric oxide synthase expression in preeclamptic (n = 3) with that in normal (n = 3) pregnancies. Frozen sections of umbilical cords, chorionic plate vessels, and terminal villi were immunostained with a monoclonal endothelial nitric oxide synthase antibody (H32).RESULTS: No difference in endothelial nitric oxide synthase immunostaining in the endothelium of the umbilical cord artery and vein, chorionic plate vessels, and stem villous vessels was found between preeclamptic and normotensive pregnancies. In contrast, in the preeclamptic placentas endothelial nitric oxide synthase immunostaining was seen in the small terminal villous vessels with underlying smooth muscle layer. In the syncytiotrophoblast endothelial nitric oxide synthase immunostaining appeared primarily apical in location and diffuse in distribution in the preeclamptic placentas but primarily basal and punctate in the normotensive placentas.CONCLUSIONS: Differences in endothelial nitric oxide synthase expression in terminal villous vessels and in syncytiotrophoblast may be a result of vascular alterations or damage that take place in the placenta in preeclampsia. These differences may alter the regulation of blood flow in the fetal and maternal placental vasculatures in preeclampsia.