CovR-controlled global regulation of gene expression in Streptococcus mutans.
CovR-controlled global regulation of gene expression in Streptococcus mutans.
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DOI：
10.1371/journal.pone.0020127
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Biswas I
中科院分区：
文献类型：
--
作者：
Dmitriev A;Mohapatra SS;Chong P;Neely M;Biswas S;Biswas I
CovR/S是一个双组分信号转导系统（TCS），在许多链球菌中控制各种毒力相关基因的表达。然而，在牙齿病原体变形链球菌中，由于同源传感器激酶CovS缺失，反应调节因子CovR似乎是一个孤儿。在这项研究中，我们探索了CovR在S. mutans中的全球转录调控。野生型菌株UA159与其等基因covR缺失菌株IBS10的转录组谱比较表明，至少128个基因（约6.5%的基因组）存在差异调控。其中69个基因在IBS10菌株中下调，59个基因在IBS10菌株中上调。突变链球菌CovR调控包括能力基因、毒力相关基因和两个基因组岛（GI）内编码的基因。由GI TnSmu2编码的基因在IBS10中被发现显著减少，而由GI TnSmu1编码的基因在突变体中被上调。通过实时RT-PCR分析进一步证实微阵列数据。此外，使用纯化的CovR蛋白进行电泳迁移转移试验，证实了一些差异表达基因的直接调控。一项蛋白质组学研究也显示了突变菌株中蛋白质表达的普遍扰动。我们的研究结果表明，CovR确实在该致病性链球菌的几个毒力相关性状的调控中发挥了重要作用。
CovR/S is a two-component signal transduction system (TCS) that controls the expression of various virulence related genes in many streptococci. However, in the dental pathogen Streptococcus mutans, the response regulator CovR appears to be an orphan since the cognate sensor kinase CovS is absent. In this study, we explored the global transcriptional regulation by CovR in S. mutans. Comparison of the transcriptome profiles of the wild-type strain UA159 with its isogenic covR deleted strain IBS10 indicated that at least 128 genes (∼6.5% of the genome) were differentially regulated. Among these genes, 69 were down regulated, while 59 were up regulated in the IBS10 strain. The S. mutans CovR regulon included competence genes, virulence related genes, and genes encoded within two genomic islands (GI). Genes encoded by the GI TnSmu2 were found to be dramatically reduced in IBS10, while genes encoded by the GI TnSmu1 were up regulated in the mutant. The microarray data were further confirmed by real-time RT-PCR analyses. Furthermore, direct regulation of some of the differentially expressed genes was demonstrated by electrophoretic mobility shift assays using purified CovR protein. A proteomic study was also carried out that showed a general perturbation of protein expression in the mutant strain. Our results indicate that CovR truly plays a significant role in the regulation of several virulence related traits in this pathogenic streptococcus.
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影响因子：
3.2
作者：
Biswas, Indranil;Drake, Laura;Biswas, Saswati
通讯作者：
Biswas, Saswati
影响因子：
3.6
作者：
Federle, MJ;Scott, JR
通讯作者：
Scott, JR
DOI：
10.1073/pnas.202353699
发表时间：
2002-10-15
影响因子：
11.1
作者：
Graham, MR;Smoot, LM;Musser, JM
通讯作者：
Musser, JM
影响因子：
3.2
作者：
Biswas, S;Biswas, I
通讯作者：
Biswas, I
影响因子：
3.2
作者：
Chaussee, MA;Callegari, EA;Chaussee, MS
通讯作者：
Chaussee, MS