Prolonged rhythmic gum chewing suppresses nociceptive response via serotonergic descending inhibitory pathway in humans
Prolonged rhythmic gum chewing suppresses nociceptive response via serotonergic descending inhibitory pathway in humans
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DOI：
10.1016/j.pain.2005.07.009
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发表时间：
2005-11-01
期刊：
影响因子：
7.4
通讯作者：
Arita, H
中科院分区：
文献类型：
--
作者：
Mohri, Y;Fumoto, M;Arita, H
5-羟色胺(5-HT)能神经元参与调控伤害性信息的传递。在动物中，5-羟色胺神经元的活动被证实通过咀嚼和运动等节律性行为而增强。因此，我们假设5-羟色胺下行抑制通路可能被人类有节律性的咀嚼口香糖行为所增强。为了评估这一想法，我们检查了伤害性屈曲反射(NFR)，同时受试者有节奏地咀嚼口香糖20分钟。电刺激腓肠神经诱发NFR，股二头肌记录诱发电位。并进行视觉模拟评分(VAS)。为了评价5-羟色胺的活性，我们用高效液相色谱法测定了血小板贫乏血浆(PPP)和全血(WB)中5-羟色胺的含量。NFR面积和VAS在咀嚼开始后5分钟显著减少，并持续到停止咀嚼。不咀嚼时，NFR和VAS无明显变化。PPP 5-羟色胺水平在停止咀嚼后立即显著升高，并在停止咀嚼30分钟后恢复到咀嚼前水平。停止咀嚼30min后Wb5-羟色胺水平显著高于咀嚼前水平。最近在血脑屏障上发现了5-羟色胺转运体，这表明血液中5-羟色胺的升高可能反映了大脑中5-羟色胺水平的增加。目前的结果支持我们的假设，即咀嚼的节律性行为通过5-羟色胺下行抑制通路抑制伤害性反应。(C)2005年国际疼痛研究协会。爱思唯尔出版，版权所有。
Serotonergic (5-HT) neurons are implicated in modulating nociceptive transmission. It is established that 5-HT neuronal activity is enhanced by rhythmic behaviors such as chewing and locomotion in animals. We thus hypothesized that 5-HT descending inhibitory pathways may be enhanced by rhythmic behavior of gum chewing in humans. To evaluate this idea, we examined nociceptive flexion reflex (NFR), while a subject chewed gum rhythmically for 20 min. NFR was elicited by electrical stimulation of the sural nerve, and the evoked potential was recorded from the biceps femoris muscle. Visual analogue scale (VAS) was also obtained. To assess 5-HT activity, we determined 5-HT levels quantitatively in platelet poor plasma (PPP) and whole blood (WB) using HPLC system. Both NFR area and VAS were significantly decreased at 5 min after the onset of chewing and these reductions persisted until cessation of chewing. There were no significant changes in NFR and VAS while resting without chewing. The PPP 5-HT level increased significantly just after cessation of chewing and had returned to the pre-chewing level by 30 min after cessation of chewing. The WB 5-HT level obtained 30 min after cessation of chewing was significantly greater than the pre-chewing level. Serotonin transporters have recently been discovered at the blood-brain barrier, suggesting that the rise in blood 5-HT may possibly reflect an increase in 5-HT level within the brain. The present results support our hypothesis that the rhythmic behavior of chewing suppresses nociceptive responses via the 5-HT descending inhibitory pathway. (c) 2005 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.