Electrical stimulation of microengineered skeletal muscle tissue: Effect of stimulus parameters on myotube contractility and maturation
Electrical stimulation of microengineered skeletal muscle tissue: Effect of stimulus parameters on myotube contractility and maturation
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DOI：
10.1002/term.2502
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发表时间：
2018-04-01
影响因子：
3.3
通讯作者：
Salehi, Sahar
中科院分区：
文献类型：
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作者：
Sadeghian, Ramin Banan;Ebrahimi, Majid;Salehi, Sahar
在体外进行工程的骨骼肌组织是自然的，缺乏正常运作所需的纤维数量，而且退化迅速。电刺激已被广泛用于弥补这种神经活动的缺乏，但刺激参数与组织反应之间的关系仍存在争议。在这里，我们研究了通宵电刺激(训练)对在微图案化明胶甲基丙烯酰基(GelMA)底物上生长的C2C12肌管的收缩和成熟度的影响。将频率、半持续时间和外加脉冲串幅度分别为f=1 Hz、t(On)=0.5ms和V-app={3V、4V、4.5V}的双极矩形脉冲(BRP)序列施加于微槽基质上形成的肌管12h。在V-APP>；=4V的整个训练期间，排列的肌管收缩。训练结束后，立即对样本进行2V(APP)的一系列BRP
Skeletal muscle tissues engineered in vitro are aneural, are short in the number of fibres required to function properly and degenerate rapidly. Electrical stimulation has been widely used to compensate for such a lack of neural activity, yet the relationship between the stimulation parameters and the tissue response is subject to debate. Here we studied the effect of overnight electrical stimulation (training) on the contractility and maturity of aligned C2C12 myotubes developed on micropatterned gelatin methacryloyl (GelMA) substrates. Bipolar rectangular pulse (BRP) trains with frequency, half-duration and applied pulse train amplitudes of f=1 Hz, t(on)=0.5 ms and V-app={3 V, 4 V, 4.5 V}, respectively, were applied for 12 h to the myotubes formed on the microgrooved substrates. Aligned myotubes were contracting throughout the training period for V-app >= 4 V. Immediately after training, the samples were subjected to series of BRPs with 2V(app)