Factors affecting poor mycelial growth on cadavers of Monochamus alternatus infectedwith an entomopathogenic fungus, Beauveria bassiana
Factors affecting poor mycelial growth on cadavers of Monochamus alternatus infectedwith an entomopathogenic fungus, Beauveria bassiana
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影响白僵菌感染松墨天牛尸体菌丝生长不良的因素
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
T. Jun
中科院分区：
文献类型：
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作者：
Shimazu Mitsuaki;T. Jun
本文研究了球孢白僵菌在松墨天牛（Monochamus alternatus）索耶成虫尸体上生长不良的寄主因素和病原菌因素。检查导致尸体菌丝生长、分生孢子密度和B萌发率不良的制剂质量。对巴西纳的配方进行了检查，但分析表明，两者都足够高，符合标准。为了考察寄主昆虫的影响因素，采用M.用该制剂接种具有各种饲养史的松墨天牛成虫。这些实验表明，真菌产生更好的菌丝体生长的成年人生长在松树上相比，那些人工饮食，并在年轻的成年人比老年人。为了检查真菌分离物的可能问题，比较了原始分离物和3个再分离物（来自在接种的成虫上具有不良菌丝体生长的制剂、来自具有不良菌丝体生长的尸体和来自通过接种原始分离物获得的完全覆盖有菌丝体的尸体）的特征。用原分离物和3个再分离物的菌丝体注射甲虫，死亡率和在寄主尸体上的菌丝生长没有差异。但在液体培养基中摇瓶培养时，菌丝体和菌丝体密度仅为原菌株和另外2个菌株的1/10。用11个随机引物进行RAPD分析，发现原始分离物和3个再分离物的基因组DNA没有差异。B菌丝生长较差。白僵菌在宿主尸体上的传播被认为是由几个因素的组合引起的，如羽化后的天数、幼虫的饮食和血腔中真菌的增殖潜力。
Factors of the host insect and of the pathogen contributing to poor mycelial growth of Beauveria bassiana on adult cadavers of Japanese pine sawyer, Monochamus alternatus (Coleoptera: Cerambycidae) were investigated. To check quality of the formulation causing cadavers with poor mycelial growth, conidial densities and germination rates of the B. bassiana formulation were inspected, but analyses revealed that both were high enough to satisfy the standard. To check factors of the host insects, M. alternatus adults with various rearing histories were inoculated with this formulation. These experiments revealed that the fungus produced better mycelial growth on the adults that were grown on pine as compared to those on an artificial diet, and on young adults than on older adults. To check possible problems of the fungus isolate, the original isolate and 3 reisolates (from the formulation bearing poor mycelial growth on the inoculated adults, from a cadaver with poor mycelial growth, and from a cadaver fully covered with mycelia obtained by inoculation of the original isolate) were compared in their characters. No difference of mortality and mycelial growth on host cadavers was found when the beetles were injected with hyphal bodies of the original and 3 reisolates. However, when shaken in liquid medium, densities of hyphal bodies and mycelia of the reisolate from the formulation was only 1/10 of the original and the other 2 reisolates. No difference in genomic DNA among the original and 3 reisolates was found by RAPD analysis with 11 primers. The poor mycelial growth of B. bassiana on host cadavers was considered to be caused by combination of several factors, such as days after eclosion, larval diet, and proliferation potential of fungus in the hemocoel.