Determination of enamel protein synthesized by recombined mouse molar tooth germs in organ culture
Determination of enamel protein synthesized by recombined mouse molar tooth germs in organ culture
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DOI：
10.1016/0003-9969(95)00120-4
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发表时间：
1996-02-01
影响因子：
3
通讯作者：
Sasaki, S
中科院分区：
文献类型：
--
作者：
Baba, T;Terashima, T;Sasaki, S
上皮-间质相互作用是牙齿形态发生的先决条件。为了研究这种相互作用，我们对16.5 d小鼠胚胎磨牙胚的内牙釉质上皮和牙乳头间质进行酶解并单独或重组培养。采用酶联免疫吸附法定量测定重组牙胚合成和分泌的特征基质蛋白。该蛋白在重组牙胚培养中检测到，而在分离牙釉质上皮中未检测到。培养至第8天，釉质蛋白含量逐渐增加。重组后的上皮雏形向成釉细胞分化，并产生成釉蛋白。
Epithelial-mesenchymal interaction is a prerequisite for tooth morphogenesis. To study this interaction, inner enamel epithelium and dental papilla mesenchyme of molar tooth germs from a 16.5-day mouse embryo were dissociated enzymatically and cultured alone or after recombination. Characteristic matrix protein synthesized and secreted by recombined tooth germ was determined quantitatively by enzyme-linked immunosorbent assay. The protein was detected in the culture of recombined tooth germ but not of dissociated enamel epithelium alone. The amount of enamel protein increased until 8 days in culture. Morphological differentiation of the recombined epithelial rudiment into ameloblasts and enamel protein production were confirmed.