[Innervation of the lachrymal gland in patients with primary Sjögren's syndrome. An immunohistopathological study].
[Innervation of the lachrymal gland in patients with primary Sjögren's syndrome. An immunohistopathological study].
复制标题
[原发性干燥综合征患者泪腺的神经支配]。
DOI：
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发表时间：
2002
影响因子：
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通讯作者：
A. Toledano
中科院分区：
文献类型：
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作者：
L. Rivas;J. Murube;A. Toledano
目的
研究了原发性干燥综合征（SS 1）患者主泪腺的组织病理学参数，分析了SS 1患者泪腺神经支配的形态学和免疫组织化学变化之间的关系，以及SS 1、非自身免疫性干燥性角结膜炎（KCS）和对照组之间的免疫病理学差异。
方法
泪腺与SS 1，KCS和对照组患者进行了活检，并采用标准的透射电子显微镜技术和免疫组化方法（血管活性肠多肽-VIP）进行检查。
结果
在SS 1患者泪腺周围结缔组织中发现了中等数量的有髓和无髓神经纤维。无髓神经纤维与腺上皮、肌上皮细胞、血管内皮、浆细胞和成纤维细胞接触。非免疫性KCS患者在数量和活性方面与SS 1相似，显示正常的形态学模式。对照组显示出更多的活跃神经纤维。
结论
因此，SS 1患者的主泪腺明确地受到中等活动和正常结构的神经支配。超微结构研究表明，与非自身免疫性KCS患者无统计学差异。免疫组织化学研究表明，SS 1和KCS患者之间的泪腺中的VIP活性相似，但与对照组相比，神经支配活性显著降低。提示SS 1患者自身免疫因素对泪腺神经支配无明显影响。
PURPOSE
Histopathological parameters of the main lachrymal gland from patients with primary Sjögren's syndrome (SS1) were investigated, and the relation between morphological and immunohistochemical changes in the innervation of lachrymal gland in patients with SS1, as well as the immunopathological differences between SS1, non-autoimmune keratoconjunctivitis sicca (KCS) and controls were analysed.
METHODS
Lachrymal glands from patients with SS1, KCS and control subjects were biopsied and examined using standard transmission electron microscopic techniques and an immunohistochemical method (vasoactive intestinal polypeptide-VIP).
RESULTS
Moderate numbers of myelinated and non-myelinated nerve fibres were found in the connective tissue around lachrymal glands in patients with SS1. Non-myelinated nerve fibres made contact with glandular epithelium, myoepithelial cells, vascular endothelium, plasma cells and fibroblasts. Patients with non-immunological KCS had similar characteristics as SS1 in number and activity, showing a normal morphological pattern. Control subjects showed a higher number of active nerve fibres.
CONCLUSION
Main lachrymal gland from patients with SS1 were therefore definitively innervated with moderate activity and normal structures. The ultrastructural study demonstrated there were no statistical differences with respect to patients with non-autoimmune KCS. Immunohistochemical studies showed a similar VIP activity in lachrymal gland between patients with SS1 and KCS, but there was a significant decrease in the innervation activity with regard to control subjects. All this implies that the autoimmunological factor in patients with SS1 does not significantly affect the lachrymal gland innervation.