Multichannel series piezoelectric quartz crystal cell sensor for real time and quantitative monitoring of the living cell and assessment of cytotoxicity.
Multichannel series piezoelectric quartz crystal cell sensor for real time and quantitative monitoring of the living cell and assessment of cytotoxicity.
复制标题
DOI：
10.1021/ac502926k
复制
发表时间：
2014-10
影响因子：
7.4
通讯作者：
Feifei Tong;Y. Lian;Huang Zhou;Xiaohong Shi;F. He
中科院分区：
文献类型：
--
作者：
Feifei Tong;Y. Lian;Huang Zhou;Xiaohong Shi;F. He
本文报道了一种新型多通道串联压电石英晶体（MSPQC）细胞传感器，用于实时监测体外活细胞。构建的传感器成功用于监测MG63骨肉瘤细胞的粘附、扩散、增殖和凋亡，并研究不同浓度的氯化钴对MG63细胞的影响。使用 MSPQC 细胞传感器进行定量实时和动态细胞分析数据。与荧光染色、显微镜形态观察等方法相比，MSPQC细胞传感器具有无创、免标记、简单、廉价、可在线监测等优点。它可以自动记录细胞的生长状态，定量评估细胞增殖和对药物的凋亡反应。它将成为获取细胞水平信息的有价值的检测和分析工具，预计未来将在细胞生物学研究或细胞毒性测试领域得到应用。
A new multichannel series piezoelectric quartz crystal (MSPQC) cell sensor for real time monitoring of living cells in vitro was reported in this paper. The constructed sensor was used successfully to monitor adhesion, spreading, proliferation, and apoptosis of MG63 osteosarcoma cells and investigate the effects of different concentrations of cobalt chloride on MG63 cells. Quantitative real time and dynamic cell analyses data were conducted using the MSPQC cell sensor. Compared with methods such as fluorescence staining and morphology observation by microscopy, the MSPQC cell sensor is noninvasive, label free, simple, cheap, and capable of online monitoring. It can automatically record the growth status of cells and quantitatively evaluate cell proliferation and the apoptotic response to drugs. It will be a valuable detection and analysis tool for the acquisition of cellular level information and is anticipated to have application in the field of cell biology research or cytotoxicity testing in the future.