Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring Profiling.
Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring Profiling.
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通过多重反应监测分析对脂肪和骨骼肌组织进行靶向脂质组学分析。
DOI：
10.1007/978-1-0716-3036-5_25
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kuang,Shihuan
中科院分区：
文献类型：
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作者：
Chen,Xiyue;Ferreira,ChristinaR;Kuang,Shihuan
脂质稳态对维持正常的细胞功能至关重要，包括膜结构完整性、细胞代谢和信号转导。脂肪组织和骨骼肌是参与脂质代谢的两个主要组织。脂肪组织可以以甘油三酯（TG）的形式储存过量的脂质，在营养不足的状态下，甘油三酯可以水解释放游离脂肪酸（FFAs）。在高能量需求的骨骼肌中，脂质作为能量生产的氧化底物，但当过载时可能导致肌肉功能障碍。根据生理需求，脂质经历了迷人的生物生成和降解周期，而脂质代谢失调已越来越多地被认为是肥胖和胰岛素抵抗等疾病的标志。因此，了解脂肪组织和骨骼肌中脂质组成的多样性和动态是很重要的。在这里，我们描述了基于脂类和脂肪酰基链特定片段的多重反应监测分析的使用，以探索骨骼肌和脂肪组织中的各种脂类。我们提供了一种详细的方法来探索性分析酰基肉碱（AC）、神经酰胺（Cer）、胆固醇酯（CE）、二酰基甘油酯（DG）、FFA、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、磷脂酰肌醇（PI）、磷脂酰丝氨酸（PS）、鞘磷脂（SM）和TG。表征脂肪组织和骨骼肌在不同生理条件下的脂质组成将为肥胖相关疾病提供生物标志物和治疗靶点。
Lipid homeostasis is critical for maintaining normal cellular functions including membrane structural integrity, cell metabolism, and signal transduction. Adipose tissue and skeletal muscle are two major tissues involved in lipid metabolism. Adipose tissue can store excessive lipids in the form of triacylglyceride (TG), which can be hydrolyzed to release free fatty acids (FFAs) under insufficient nutrition states. In the highly energy-demanding skeletal muscle, lipids serve as oxidative substrates for energy production but can cause muscle dysfunction when overloaded. Lipids undergo fascinating cycles of biogenesis and degradation depending on physiological demands, while dysregulation of lipid metabolism has been increasingly recognized as a hallmark of diseases such as obesity and insulin resistance. Thus, it is important to understand the diversity and dynamics of lipid composition in adipose tissue and skeletal muscle. Here, we describe the use of multiple reaction monitoring profiling, based on lipid class and fatty acyl chain specific fragmentation, to explore various classes of lipids in skeletal muscle and adipose tissues. We provide a detailed method for exploratory analysis of acylcarnitine (AC), ceramide (Cer), cholesteryl ester (CE), diacylglyceride (DG), FFA, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylserine (PS), sphingomyelin (SM), and TG. Characterization of lipid composition within adipose tissue and skeletal muscle under different physiological situations will provide biomarkers and therapeutic targets for obesity-related diseases.