NEURONAL PP60C-SRC CONTAINS A 6-AMINO ACID INSERTION RELATIVE TO ITS NONNEURONAL COUNTERPART
NEURONAL PP60C-SRC CONTAINS A 6-AMINO ACID INSERTION RELATIVE TO ITS NONNEURONAL COUNTERPART
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DOI：
10.1126/science.2440106
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发表时间：
1987-07-24
期刊：
影响因子：
56.9
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
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作者：
MARTINEZ, R;MATHEYPREVOT, B;BALTIMORE, D
神经元细胞表达pp 60 c-src变体，该变体相对于在非神经元来源的组织中表达的pp 60 c-src显示改变的电泳迁移率和不同的V8肽模式。为了确定pp 60 c-src的神经元形式是否由脑特异性信使RNA编码，用鸡c-src探针筛选小鼠脑互补DNA（cDNA）文库，并分离3.8-丝氨酸蛋白酶c-src cDNA克隆。该克隆编码一种60千道尔顿的蛋白质，该蛋白质与鸡或人pp 60 c-src的不同之处主要在于在该分子的NH 2-末端16千道尔顿内具有6个额外的氨基酸（Arg-Lys-Val-Asp-Val-Arg）。S1核酸酶保护分析证实，脑c-src RNA在额外的6个氨基酸的位置上含有18个核苷酸的插入。这种插入发生在与鸡和人c-src基因中的剪接点相对应的位置。分离的c-src cDNA克隆编码的蛋白质显示出与从神经元来源的组织中分离的pp 60 csrc中观察到的相同的V8肽模式。
Neuronal cells express a pp60c-src variant that displays an altered electrophoretic mobility and a different V8 peptide pattern relative to pp60c-src expressed in tissues of non-neuronal origin. To determine whether the neuronal form of pp60c-src is encoded by a brain-specific messenger RNA, a mouse brain complementary DNA (cDNA) library was screened with a chicken c-src probe and a 3.8-kilobase c-src cDNA clone was isolated. This clone encodes a 60-kilodalton protein that differs from chicken or human pp60c-src primarily in having six extra amino acids (Arg-Lys-Val-Asp-Val-Arg) within the NH2-terminal 16 kilodaltons of the molecule. S1 nuclease protection analysis confirmed that brain c-src RNA contains an 18-nucleotide insertion at the position of the extra six amino acids. This insertion occurs at a position that corresponds to a splice junction in the chicken and human c-src genes. The isolated c-src cDNA clone encodes a protein that displays an identical V8 peptide pattern to that observed in pp60csrc isolated from tissues of neuronal origin.