Confirming the functional importance of a protein-DNA interaction.
Confirming the functional importance of a protein-DNA interaction.
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DOI：
10.1101/pdb.top070060
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发表时间：
2012-07-01
影响因子：
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通讯作者：
Smale, Stephen T
中科院分区：
文献类型：
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作者：
Carey, Michael F;Peterson, Craig L;Smale, Stephen T
识别与感兴趣的控制区相互作用的DNA结合蛋白已经变得非常简单。然而，一个给定的蛋白质-DNA相互作用的功能相关性是很难建立的。相互作用是相关的假设可以通过几个不同的实验进行测试，本文概述了其中的12个。必须记住，这些实验本身都不是决定性的。从每种方法获得的信息进行了描述，并解释了为什么每个产量有用，但不确定的结果。这些方法在所需努力的数量和所获得信息的质量方面差别很大。
Identifying DNA-binding proteins that interact with a control region of interest has become quite straightforward. However, the functional relevance of a given protein-DNA interaction is difficult to establish. The hypothesis that an interaction is relevant can be tested by several different experiments, 12 of which are outlined in this article. It must be remembered that none of these experiments by itself is conclusive. The information gained from each approach is described and explanations are given for why each yields useful but inconclusive results. The approaches vary widely with respect to the amount of effort required and the quality of information obtained.