Hypoxic conditions differentially regulate TAZ and YAP in cancer cells.
Hypoxic conditions differentially regulate TAZ and YAP in cancer cells.
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DOI：
10.1016/j.abb.2014.07.024
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发表时间：
2014-11-15
影响因子：
3.9
通讯作者：
Xu, Yan
中科院分区：
文献类型：
--
作者：
Yan, Libo;Cai, Qingchun;Xu, Yan
关键词：
在许多人类癌症中，河马-YAP通路被改变并被认为是一种致癌信号通路。缺氧是促进肿瘤发生的重要微环境因素。然而，低氧对河马YAP的两个最重要的效应因子YAP(Yes-Association Protein)和TAZ(转录共激活因子with PDZ-Binding Motif)的影响尚未见报道。在这项工作中，我们证明了TAZ在功能上参与了上皮性卵巢癌(EOC)或人卵巢表面上皮(HOSE)细胞的增殖和/或迁移。低氧条件(1%O2或低氧模拟物)诱导EOC细胞株OVCAR5和SKOV3的YAP磷酸化(S127)和总YAP表达减少。然而，这些条件上调了EOC细胞中S69磷酸化的TAZ的水平。已知的TAZ激酶Lats1和Akt不太可能参与低氧条件下pTAZ的上调。总之，我们的数据揭示了TAZ和YAP在低氧条件下对癌细胞的新的和不同的调节机制。
The Hippo-YAP pathway is altered and implicated as an oncogenic signaling pathway in many human cancers. Hypoxia is an important microenvironmental factor that promotes tumorigenesis. However, the effects of hypoxia on the two most important Hippo-YAP effectors, YAP (Yes-associated protein) and TAZ (transcriptional co-activator with PDZ-binding motif), have not been reported. In this work, we demonstrated that TAZ was functionally involved in cell proliferation and/or migration in epithelial ovarian cancer (EOC) or human ovarian surface epithelial (HOSE) cells. Hypoxic conditions (1% O2 or hypoxia mimics) induced a reduction of YAP phosphorylation (S127) and total YAP expression in EOC cell lines OVCAR5 and SKOV3. However, these conditions up-regulated levels of S69 phosphorylated TAZ in EOC cells. The known TAZ kinases, Lats1 and Akt, were unlikely to be involved in up-regulated pTAZ by hypoxic conditions. Together, our data revealed new and differential regulating mechanisms of TAZ and YAP in cancer cells by hypoxia conditions.
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影响因子：
11.2
作者：
Li W;Wang L;Katoh H;Liu R;Zheng P;Liu Y
通讯作者：
Liu Y
影响因子：
--
作者：
Jeong, Geun Ok;Shin, Sang Hun;Kim, Jae Ho
通讯作者：
Kim, Jae Ho
DOI：
10.2106/00004623-199072040-00010
发表时间：
1990-04-01
影响因子：
5.3
作者：
BRADFORD, DS;BOACHIEADJEI, O
通讯作者：
BOACHIEADJEI, O
影响因子：
64.5
作者：
Yu FX;Zhao B;Panupinthu N;Jewell JL;Lian I;Wang LH;Zhao J;Yuan H;Tumaneng K;Li H;Fu XD;Mills GB;Guan KL
通讯作者：
Guan KL
影响因子：
8.4
作者：
de Cristofaro, Tiziana;Di Palma, Tina;Zannini, Mariastella
通讯作者：
Zannini, Mariastella