Single-molecule measurement of strand breaks on large DNA induced by atmospheric pressure plasma jet
Single-molecule measurement of strand breaks on large DNA induced by atmospheric pressure plasma jet
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DOI：
10.1063/1.3660581
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发表时间：
2011-11
影响因子：
4
通讯作者：
H. Kurita;T. Nakajima;H. Yasuda;K. Takashima;A. Mizuno;John I. B. Wilson;S. Cunningham
中科院分区：
文献类型：
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作者：
H. Kurita;T. Nakajima;H. Yasuda;K. Takashima;A. Mizuno;John I. B. Wilson;S. Cunningham
我们报告了一个基于单分子的分析大气压等离子体射流诱导的大DNA分子链断裂。我们将DNA溶液暴露在氩等离子体射流中;然后使用涉及分子梳理的单分子观察来测量单个DNA分子的长度。DNA长度的测定表明，等离子体暴露引起DNA分子长度的显着变化。使用一个简单的数学模型来确定大的随机卷曲的DNA分子上的等离子体诱导的链断裂速率。对于大DNA分子上的链断裂，估计了速率。
We report a single-molecule-based analysis of strand breakages on large DNA molecules induced by an atmospheric pressure plasma jet. We exposed DNA solution to an argon plasma jet; single-molecule observation that involved molecular combing was then used to measure the length of individual DNA molecules. The measuredDNA length showed that plasma exposure caused a marked change in length of DNA molecules. The rate of plasma-induced strand breakage on large random-coiled DNA molecules was determined using a simple mathematical model. For strand breaks on large DNA molecules the rate was estimated.