Electrical stimulation accelerates and increases expression of BDNF and trkB rnRNA in regenerating rat femoral motoneurons
Electrical stimulation accelerates and increases expression of BDNF and trkB rnRNA in regenerating rat femoral motoneurons
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DOI：
10.1046/j.1460-9568.2000.01341.x
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发表时间：
2000-12-01
影响因子：
3.4
通讯作者：
Gordon, T
中科院分区：
文献类型：
--
作者：
Al-Majed, AA;Brushart, TM;Gordon, T
电刺激促进运动轴突再生的速度和准确性。刺激的积极作用是在细胞体中介导的。在这里，我们的特点的影响，电刺激对运动神经元的BDNF及其受体，trkB，两个基因的表达水平在运动神经元与再生和调节的电活动在各种神经元。我们采用半定量原位杂交技术，测量编码BDNF和全长trkB受体的mRNA表达的时间间隔为8小时，2天和7天后，单侧股神经切断，缝合，和刺激。再生运动神经元中的表达与对侧完整运动神经元中的表达进行比较。股神经缝合和假刺激后8 h和2 d BDNF和trkB信号均未显著上调。到第7天，BDNF和trkB mRNA表达均增加2倍。与此相反，刺激切割和修复的神经仅1小时，导致BDNF和trkB mRNA的快速上调3倍和2倍，分别在第一个8小时。刺激效果在第2天达到峰值，信号分别增加6倍和4倍。此后，BDNF和trkB mRNA的表达水平下降到等于神经修复和假刺激后7天观察到的2倍增加。我们的结论是，短暂的电刺激刺激BDNF和trkB的表达再生运动神经元。由于电刺激已知加速轴突再生，我们认为BDNF和trkB表达的变化与轴突再生的加速相关。
Electrical stimulation promotes the speed and accuracy of motor axonal regeneration. The positive effects of stimulation are mediated at the cell body. Here we characterize the effect of electrical stimulation on motoneuronal expression of BDNF and its receptor, trkB, two genes whose expression levels in motoneurons correlate with regeneration and are regulated by electrical activity in a variety of neurons. We used semiquantitative in situ hybridization to measure expression of mRNA encoding BDNF and the full-length trkB receptor at intervals of 8 h, 2 days and 7 days after unilateral femoral nerve cut, suture, and stimulation. Expression in regenerating motoneurons was compared to that of contralateral intact motoneurons. BDNF and trkB signals were not significantly upregulated 8 h and 2 days after femoral nerve suture and sham stimulation. By 7 days, there was a 2-fold increase in both BDNF and trkB mRNA expression. In contrast, stimulation of cut and repaired nerves for only 1 h led to rapid upregulation of BDNF and trkB mRNA by 3-fold and 2-fold, respectively, within the first 8 h. The stimulation effect peaked at 2 days with 6-fold and 4-fold increases in the signals, respectively. Thereafter, the levels of BDNF and trkB mRNA expression declined to equal the 2-fold increase seen at 7 days after nerve repair and sham-stimulation. We conclude that brief electrical stimulation stimulates BDNF and trkB expression in regenerating motoneurons. Because electrical stimulation is known to accelerate axonal regeneration, we suggest that changes in the expression of BDNF and trkB correlate with acceleration of axonal regeneration.