Development and host cell modifications of Plasmodium falciparum blood stages in four dimensions
Development and host cell modifications of Plasmodium falciparum blood stages in four dimensions
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DOI：
10.1038/ncomms1169
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发表时间：
2011-01-01
影响因子：
16.6
通讯作者：
Spielmann, Tobias
中科院分区：
文献类型：
--
作者：
Gruering, Christof;Heiber, Arlett;Spielmann, Tobias
恶性疟原虫的血液阶段导致疟疾的病理；然而，寄生虫在生命周期的这一复杂部分的发展从未被可视化。在这项研究中，我们首次使用四维成像来显示红细胞中单个寄生虫的发展以及伴随而来的宿主细胞的修饰。我们的数据可视化了一种出人意料的动态寄生虫，为这个生命周期阶段提供了参考，并挑战了恶性疟原虫中蛋白质输出与宿主细胞修饰的生物发生有关的模型，称为毛雷尔裂隙。我们的结果为疟疾原虫的血液期发育、毛雷尔裂隙发育和蛋白质输出提供了一个新的视角，并为研究动态过程、药物效应和突变体的表型打开了大门。
Blood stages of Plasmodium falciparum cause the pathology of malaria; however, the progression of the parasite through this complex part of the life cycle has never been visualized. In this study, we use four-dimensional imaging to show for the first time the development of individual parasites in erythrocytes and the concomitant host cell modifications. Our data visualize an unexpectedly dynamic parasite, provide a reference for this life cycle stage and challenge the model that protein export in P. falciparum is linked to the biogenesis of host cell modifications termed Maurer's clefts. Our results provide a novel view of the blood-stage development, Maurer's cleft development and protein export in malaria parasites, and open the door to study dynamic processes, drug effects and the phenotype of mutants.