Electroporation Formulation for Cell Therapy
Electroporation Formulation for Cell Therapy
复制标题
DOI：
10.1007/978-1-4614-9632-8_4
复制
发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Li, Shulin
中科院分区：
文献类型：
--
作者：
Hu, Jiemiao;Li, Shulin
细胞转染效率往往决定了基于细胞的基因治疗的成功。通过Nucleofector技术的细胞转染产生高转染效率和低细胞毒性。然而，由于商业秘密，每种缓冲液中的组分是未知的，这不仅增加了电穿孔研究的成本，而且限制了Nucleofector在临床细胞基因治疗中的应用。因此，我们开发了一种三步法来确定电穿孔用于多种癌症和干细胞系的最佳条件，包括缓冲液，程序和附加聚合物。这种方法可以降低成本，使研究人员能够找到他们感兴趣的细胞系的最佳电穿孔条件，并大大提高电穿孔在临床细胞为基础的基因治疗的应用潜力。
Cell transfection efficiency often determines the success of cell-based gene therapy. Cell transfection via Nucleofector technology yields high transfection efficiency and low cytotoxicity. However, owing to trade secrecy, the components in each buffer are unknown, which not only increases the cost of electroporation studies but also limits the application of Nucleofector in clinical cell-based gene therapies. Thus, we developed a three-step method to determine the optimal conditions, including buffer, program, and additional polymer, in electroporation for multiple cancers and stem cell lines. This method could reduce the cost, allow researchers to find the optimal electroporation conditions for their cell lines of interest, and greatly boost the application potential of electroporation in clinical cell-based gene therapies.