Characterization of Mg2+-induced conformational change in the 50S ribosomal subunit by differential hydrogen exchange.
Characterization of Mg2+-induced conformational change in the 50S ribosomal subunit by differential hydrogen exchange.
复制标题
通过差异氢交换表征 50S 核糖体亚基中 Mg2 诱导的构象变化。
DOI：
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发表时间：
1980
影响因子：
14.9
通讯作者：
G. Hui Bon Hoa
中科院分区：
文献类型：
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作者：
D. Bonnet;E. Bégard;M. Grunberg‐Manago;G. Hui Bon Hoa
当镁浓度降低到核糖体活性完全保留的范围内时，差分氢交换技术允许检测大肠杆菌核糖体50S亚基的构象变化。这种变化的特点是，在Mg 2+从10 mM跳到2 mM的情况下，大约30个不稳定的氢加速了70倍。所涉及的氢的数量很少，这可以解释用其他方法检测这种变化的困难。
The technique of differential hydrogen exchange allows detection of a conformational change in the 50S subunit of Escherichia coli ribosome when the magnesium concentration is lowered in a range where ribosomal activity is fully preserved. This change is characterized by a seventy-fold acceleration of about thirty labile hydrogens in the case of a Mg2+ jump from 10 mM to 2 mM. The small number of hydrogens involved can explain the difficulty in detecting this change by other methods.