Phosphate-perylene modified G-quadruplex probes for the detection of Pb2+ using fluorescence anisotropy
Phosphate-perylene modified G-quadruplex probes for the detection of Pb2+ using fluorescence anisotropy
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磷酸盐-苝修饰的 G-四联体探针用于利用荧光各向异性检测 Pb2
DOI：
10.1039/c6tb00539j
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发表时间：
2016-01-01
影响因子：
7
通讯作者：
Tang, Xinjing
中科院分区：
文献类型：
--
作者：
Wang, Zhixuan;Pei, Xiaojing;Tang, Xinjing
一种简单通用的磷酸-苝修饰策略被应用于开发具有凝血酶结合适体（TBA）和[d（TGGGT）（4）]（G4）序列的G-四链体探针。在寡核苷酸的不同磷酸位置插入一个二萘嵌苯部分，对G-四链体结构没有显着影响。当与K+或Pb 2+结合时，由于标记位置和G-四链体结构的不同，这些探针显示出不同的荧光各向异性响应。两种探针（G4-9和TBA-9）通过荧光各向异性成功地用于Pb 2+的检测。当Pb ~（2+）与G4-9或TBA-9形成复合物后，苝的旋转扩散受到限制，荧光各向异性显著增强。两种探针对Pb 2+均表现出良好的灵敏度，其荧光各向异性信号对Pb 2+浓度的对数表现出良好的线性响应，TBA-9和G4-9的检测限分别为24.5 nM和30.0 nM。
A simple and universal phosphate-perylene modification strategy was applied in the development of G-quadruplex probes with thrombin binding aptamer (TBA) and [d(TGGGT)(4)] (G4) sequences. A perylene moiety was inserted at different phosphate positions of oligonucleotides without a significant effect on the G-quadruplex structures. Upon binding with K+ or Pb2+, these probes showed different perylene fluorescence anisotropy responses due to the different labeling positions and G-quadruplex structures. Two probes (G4-9 and TBA-9) were successfully used in Pb2+ detection through fluorescence anisotropy. Once the complexes of Pb2+ with G4-9 or TBA-9 were formed, the rotational diffusion of the perylene moiety was limited, resulting in a significant increase in fluorescence anisotropy. Both probes showed good sensitivity to Pb2+ and their fluorescence anisotropy signals demonstrated good linear responses to the logarithm of Pb2+ concentrations and the detection limits were 24.5 nM and 30.0 nM for TBA-9 and G4-9, respectively.