Survey of Anaplasmataceae bacteria in sheep from Senegal
Survey of Anaplasmataceae bacteria in sheep from Senegal
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DOI：
10.1007/s11250-013-0399-y
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发表时间：
2013-10-01
影响因子：
1.7
通讯作者：
Ndiaye, Mady
中科院分区：
文献类型：
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作者：
Djiba, Mamadou Lamine;Mediannikov, Oleg;Ndiaye, Mady
作者研究了无形体科细菌在西非绵羊急性疾病中的作用，并对塞内加尔两个地区的120只发热羊进行了检查和抽样。用两对引物（groEL引物和16 SrRNA引物）对采集的血样进行PCR检测，镜检发现52/120例标本存在红细胞内和/或吞噬细胞内的球形包涵体。在48/52例病例中，我们成功地鉴定了细菌病原体：38例为绵羊无形体; 6例为反刍埃立克体; 2例为嗜吞噬细胞无形体; 1例为扁平无形体; 1例为与无形体密切相关的未培养无形体。我们的研究证明了临床患病绵羊血液中来自无形体科的致病菌种类繁多。A. ovis被意外地频繁识别。第一次，A。在撒哈拉以南非洲发现了嗜吞噬细胞菌，现在可以重新考虑其进一步的流行病学。犬病原A.扁平，但未描述的无形体属“Badiour”，在绵羊病理学应更密切地研究。
The authors studied the role of bacteria belonging to Anaplasmataceae family as the causes of acute illnesses of sheep in West Africa.We examined and sampled 120 febrile sheep in two regions of Senegal for this study. The DNA extracted from these blood samples was tested by PCR using two pairs of primers (groEL-based and 16S rRNA gene-based).In 52/120 samples, the microscopic examination revealed intraerythrocytic and/or intraphagocytic spherical inclusions. In 48/52 cases, we succeeded in identifying the bacterial agent: in 38 cases, it was Anaplasma ovis; in six cases, it was Ehrlichia ruminantium; in two cases, Anaplasma phagocytophilum; in one case, Anaplasma platys; and in one case, a yet uncultured Anaplasma sp. closely related to A. phagocytophilum.Our studies demonstrated the great variety of pathogenic bacteria from the Anaplasmataceae family in the blood of clinically ill sheep. A. ovis was identified unexpectedly often. For the first time, A. phagocytophilum was found in sub-Saharan Africa, and its further epidemiology may be now reconsidered. The roles of canine pathogen, A. platys, and yet undescribed Anaplasma sp. "Badiour," in ovine pathology should be more closely studied.