Monitoring of Lawsonia intracellularis in breeding herd gilts
Monitoring of Lawsonia intracellularis in breeding herd gilts
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DOI：
10.1016/j.vetmic.2009.09.034
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发表时间：
2010-05-19
影响因子：
3.3
通讯作者：
Wallgren, P.
中科院分区：
文献类型：
--
作者：
Jacobson, M.;Aspan, A.;Wallgren, P.
在现代养猪生产中，增殖性肠病是幼龄动物腹泻和生长不良的常见原因。本研究旨在确定胞内劳森氏菌通过后备母猪销售传播的可能性以及通过适当的生物安全措施保护牛群的可能性。这是通过从分娩畜栏的最后一天至销售期间，对感染繁殖畜群中的50头后备母猪进行重复采样来实现的。此外，在受体猪群隔离期间，对该猪群出售的60头后备母猪进行了检测。为了确认无感染，还对受体猪群中的100头生长猪进行了检测。分别通过PCR和ELISA分析个体粪便（n = 748）和血液（n = 728）样本。未证实胞内乳杆菌从母猪传播至其后代。然而，证明了后备后备母猪在牛群之间的可能传播。在12和15周龄时出现散毒高峰，在繁殖牛群和受体牛群的隔离检疫中，24-36周龄时单只动物也呈PCR阳性。此外，还提示可能存在慢性感染的带菌者动物。虽然胞内乳杆菌广泛传播，但似乎可以通过采取适当的生物安全措施来避免畜群之间的传播。因此，建议在育种畜群中建立畜群概况，以避免出售受感染的动物，并确定受体畜群的健康状况。此外，应了解受体畜群的健康状况。(C)2009爱思唯尔有限公司版权所有。
In modern pig production, proliferative enteropathy is a common cause of diarrhoea and poor growth in young animals. This study aimed to determine the possible spread of Lawsonia intracellularis through the sale of replacement gilts and the possibility to protect the herds by adequate biosecurity measures. This was achieved by repeated sampling of 50 gilts in an infected multiplying herd, from the last day in the farrowing pen and until sale. Further, 60 gilts sold from this herd were tested during their stay in quarantine in a recipient herd. To confirm freedom from infection, 100 growing pigs in the recipient herd were also tested. Individual faecal (n = 748) and blood (n = 728) samples were analysed by PCR and ELISA, respectively. Transmission of L intracellularis from the sows to their offspring was not demonstrated. However, the possible transmission between herds by replacement gilts was demonstrated. Peak shedding occurred at 12 and 15 weeks of age, and single animals were also PCR-positive at 24-36 weeks of age in the multiplying herd and in the quarantine in the recipient herd. Further, the possible occurrence of chronically infected carrier animals was suggested. Although L intracellularis is widely spread, it appears possible to avoid the transmission between herds by employing adequate biosecurity measures. Thus, it would be advisable to establish herd profiles in breeding herds to avoid the selling of infected animals as well as to establish the health status of the recipient herd. Further, the health status of the recipient herds should be known. (C) 2009 Elsevier B.V. All rights reserved.