CONSTITUTIVE EXPRESSION OF A 2',5'-OLIGOADENYLATE SYNTHETASE CDNA RESULTS IN INCREASED ANTIVIRAL ACTIVITY AND GROWTH SUPPRESSION
CONSTITUTIVE EXPRESSION OF A 2',5'-OLIGOADENYLATE SYNTHETASE CDNA RESULTS IN INCREASED ANTIVIRAL ACTIVITY AND GROWTH SUPPRESSION
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DOI：
10.1089/jir.1989.9.649
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发表时间：
1989-12-01
期刊：
影响因子：
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通讯作者：
WILLIAMS, BRG
中科院分区：
文献类型：
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作者：
RYSIECKI, G;GEWERT, DR;WILLIAMS, BRG
干扰素（IFN）诱导的酶2''，5'' - 寡腺苷酸（2-5a）合成酶已经与人和动物细胞中抗病毒活性的发展有关。但是，其在IFN介导的生长抑制中的作用尚不清楚。为了阐明2-5a合成酶的功能，我们稳定地将人2-5A合成酶cDNA引入了人胶质母细胞瘤细胞系（T98G）。这些细胞中cDNA的组成型表达与脑膜炎病毒对感染的耐药性水平增加有关。一个转染的亚克隆表达了2-5a合成酶活性的水平升高，也显示出细胞增殖的速度降低。
The interferon (IFN)-induced enzyme 2'',5''-oligoadenylate (2-5A) synthetase has been implicated in the development of antiviral activity in human and animal cells. However, its role in IFN-mediated growth inhibition remains unclear. To elucidate the function of 2-5A synthetase, we have stably introduced a human 2-5A synthetase cDNA into a human glioblastoma cell line (T98G). Constitutive expression of the cDNA in these cells is associated with increased levels of resistance to infection by encephalomyocarditis virus. One transfected subclone, which expresses elevated levels of 2-5A synthetase enzyme activity, also shows a reduced rate of cellular proliferation.