Chimeric Dr fimbriae with a herpes simplex virus type 1 epitope as a model for a recombinant vaccine.
Chimeric Dr fimbriae with a herpes simplex virus type 1 epitope as a model for a recombinant vaccine.
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具有 1 型单纯疱疹病毒表位的 Chimeric Dr fimbriae 作为重组疫苗的模型。
DOI：
10.1128/iai.71.10.5505-5513.2003
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发表时间：
2003
影响因子：
3.1
通讯作者：
Kur,Józef
中科院分区：
文献类型：
--
作者：
Zalewska,Beata;Piatek,Rafał;Konopa,Grazyna;Nowicki,Bogdan;Nowicki,Stella;Kur,Józef
The potential of the major structural protein DraE ofEscherichia coliDr fimbriae has been used to display an 11-amino-acid peptide of glycoprotein D derived from herpes simplex virus (HSV) type 1. The heterologous sequence mimicking an epitope from glycoprotein D was inserted in one copy into thedraEgene in place of a predicted 11-amino-acid sequence in the N-terminal region of surface-exposed domain 2 within the conserved disulfide loop (from Cys21 to Cys53). The inserted epitope was displayed on the surface of the chimeric DraE protein as evidenced by immunofluorescence and was recognized by monoclonal antibodies to the target HSV glycoprotein D antigen. Conversely, immunization of rabbits with purified chimeric Dr-HSV fimbriae resulted in a serum that specifically recognized the 11-amino-acid epitope of HSV glycoprotein D, indicating the utility of the strategy employed.
