Growth of tumour cell lines in polymer capsules: ultrastructure of encapsulated PC12 cells.
Growth of tumour cell lines in polymer capsules: ultrastructure of encapsulated PC12 cells.
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肿瘤细胞系在聚合物胶囊中的生长：封装的 PC12 细胞的超微结构。
DOI：
10.1007/bf01186951
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Greene,LA
中科院分区：
文献类型：
--
作者：
Jaeger,CB;Aebischer,P;Tresco,PA;Winn,SR;Greene,LA
最近的研究表明，聚合物封装的 PC12 细胞释放足够量的多巴胺，可显着改变多巴胺能中脑神经元单侧损伤的动物的行为模式。由于细胞精细结构为评估储存功能、胞吐作用、代谢、细胞活性和细胞活力提供了有用的衡量标准，因此我们检查了在体外维持或植入大鼠或豚鼠前脑的半透性聚合物胶囊中生长的 PC12 细胞的超微结构。封装的 PC12 细胞在整个六个月的评估期内保持活力并继续分裂。两种环境中封装的 PC12 细胞的总体形态相似，并且类似于单层培养物中生长的 PC12 细胞。在短期培养中，封装的 PC12 细胞通常含有大量的嗜铬细胞样颗粒。封装的细胞最初在其表面具有丰富的微绒毛，随着时间的推移，微绒毛的频率逐渐下降。长期封闭十周或更长时间后，体外培养的胶囊和脑植入胶囊中细胞的细胞质中检测到较少的分泌颗粒。植入的胶囊中的一些细胞具有细长的丝状伪足，而培养的胶囊中的 PC12 细胞则不存在这种细长的丝状伪足。 PC12细胞的形态变化可能与生长条件的改变有关，例如血清和氧气浓度、不同环境中生长因子的存在或不存在，以及与细胞密度相关的细胞相互作用的变化以及胶囊内碎片随时间的积累。由于多巴胺能 PC12 嗜铬细胞瘤细胞在半透性聚合物胶囊中至少可以存活六个月，因此这种“细胞胶囊”可以为多巴胺替代疗法中分泌多巴胺的胚胎神经移植物提供替代方案。
Recent studies indicate that polymer-encapsulated PC12 cells release sufficient amounts of dopamine to significantly alter behavioural paradigms in animals with unilateral lesions of dopaminergic midbrain neurons. Because cell fine structure provides a useful measure for assessment of storage function, exocytosis, metabolism, cell activity and cell viability, we examined the ultrastructure of PC12 cells grown in semi-permeable polymer capsules maintainedin vitroor implanted into the forebrain of rats or guinea pigs. Encapsulated PC12 cells remained viable and continued to divide for the entire evaluation period of six months. Overall morphologies of encapsulated PC12 cells were similar in both environments and they resembled PC12 cells grown in monolayer cultures. In short-term cultures, encapsulated PC12 cells typically contained abundant quantities of chromaffin cell-like granules. The encapsulated cells had initially abundant microvilli on their surfaces which decline in frequency over time. After long-term enclosure for ten weeks or more, fewer secretory granules were detected in the cytoplasm of cells in capsules culturedin vitroand in brain-implated capsules. Some cells in implanted capsules had long slender filipodia that were not present on PC12 cells in cultured capsules. The morphological changes of PC12 cells may correlate with altered growth conditions such as serum and oxygen concentrations, the presence or absence of growth factors in different environments, and with changes of cell interactions related to cell densities and build up of debris within the capsules over time. Since dopaminergic PC12 pheochromocytoma cells remain viable in semi-permeable polymer capsules for at least six months, such ‘cell-capsules’ could provide an alternative to dopamine-secreting embryonic neural grafts in dopamine replacement therapies.