Neural circuits mediate electrosensory behavior in Caenorhabditis elegans
Neural circuits mediate electrosensory behavior in Caenorhabditis elegans
复制标题
DOI：
10.1523/jneurosci.0775-07.2007
复制
发表时间：
2007-07-11
影响因子：
5.3
通讯作者：
Samuel, Aravinthan D. T.
中科院分区：
文献类型：
--
作者：
Gabel, Christopher V.;Gabel, Harrison;Samuel, Aravinthan D. T.
线虫秀丽隐杆线虫故意向电场中的负极爬行。通过量化单个蠕虫在电场中的运动，我们发现线虫在持续向前运动的过程中更喜欢以与电场方向特定的角度爬行，并且首选角度与场强成正比。线虫通过突然转向和反转来响应随时间变化的电场而重新定向，这是线虫在其他运动行为模式中使用的标准重新定向动作。突变或激光烧蚀会破坏两栖类感觉神经元的结构和功能，也会破坏电感觉行为。通过对固定蠕虫的两栖动物感觉神经元之间的细胞内钙动态进行成像，我们发现特定的两栖动物感觉神经元对电场的方向和强度敏感。我们通过表明特定的中间神经元影响电感应转向过程中突然转向和反转的利用，将我们的分析扩展到运动水平。因此，电感觉行为可以用作模型系统，用于理解线虫神经系统如何将感觉输入转化为运动输出。
The nematode Caenorhabditis elegans deliberately crawls toward the negative pole in an electric field. By quantifying the movements of individual worms navigating electric fields, we show that C. elegans prefers to crawl at specific angles to the direction of the electric field in persistent periods of forward movement and that the preferred angle is proportional to field strength. C. elegans reorients itself in response to time-varying electric fields by using sudden turns and reversals, standard reorientation maneuvers that C. elegans uses during other modes of motile behavior. Mutation or laser ablation that disrupts the structure and function of amphid sensory neurons also disrupts electrosensory behavior. By imaging intracellular calcium dynamics among the amphid sensory neurons of immobilized worms, we show that specific amphid sensory neurons are sensitive to the direction and strength of electric fields. We extend our analysis to the motor level by showing that specific interneurons affect the utilization of sudden turns and reversals during electrosensory steering. Thus, electrosensory behavior may be used as a model system for understanding how sensory inputs are transformed into motor outputs by the C. elegans nervous system.