Fine mapping of the dialytic gene that controls multicellular trichome formation and stamen development in tomato
Fine mapping of the dialytic gene that controls multicellular trichome formation and stamen development in tomato
复制标题
控制番茄多细胞毛状体形成和雄蕊发育的透析基因的精细定位
DOI：
10.1007/s00122-016-2722-2
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发表时间：
2016-08-01
影响因子：
5.4
通讯作者：
Yang, Changxian
中科院分区：
文献类型：
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作者：
Chang, Jiang;Yu, Ting;Yang, Changxian
利用图位克隆，我们划定了透析基因的一个约109-kb的片段，控制多细胞毛状体的形成和雄蕊发育番茄。毛状体存在于几乎所有陆生植物的表皮中，包括单细胞和多细胞类型。拟南芥单细胞毛状体的分子机制已被很好地表征。然而，关于番茄（Solanum lycopersicum）多细胞毛状体的调控途径的知识有限。表型分析的透析（dl）突变体LA 3724表明，毛状体分叉和雄蕊不关闭。为了克隆和表征dl，我们使用来自dl突变体的两个杂交组合的两个F2群体：LA 3724 × IL 8 -1和LA 3724 × LA 1589（茄属pimpinellifolium），将该基因定位到约109-kb的片段。在这项研究中使用了两种类型的分子标记，包括切割扩增多态性序列和插入-缺失事件。序列分析预测存在7个推定的开放阅读框架，包括两个未知蛋白，两个磷脂酶Ds，糖基水解酶家族5蛋白/纤维素，胆碱/乙醇胺激酶，和水通道蛋白样蛋白。dl突变体中类水通道蛋白基因表达明显上调。因此，我们推断该基因是表型的潜在候选者。研究结果为阐明番茄毛状体形成和雄蕊发育的调控途径奠定了基础。
Using map-based cloning, we delimited the dialytic gene to an approximately 109-kb fragment, which controls multicellular trichome formation and stamen development in tomato. Trichomes exist in the epidermis of nearly all terrestrial plants, including unicellular and multicellular types. The molecular mechanism of unicellular trichomes in Arabidopsis is well characterized. However, knowledge about the regulatory pathway of multicellular trichomes in tomato (Solanum lycopersicum) is limited. Phenotypic analysis of the dialytic (dl) mutant LA3724 demonstrated that the trichomes are forked and the stamens are unclosed. To clone and characterize dl, we mapped this gene to an approximately 109-kb fragment using two F2 populations derived from the two crosses of dl mutant: LA3724 × IL8-1 and LA3724 × LA1589 (Solanum pimpinellifolium). Two types of molecular markers were utilized in this study, including cleaved amplified polymorphic sequences and insertion-deletion events. Sequence analysis predicted the presence of seven putative open reading frames, including two unknown proteins, two phospholipase Ds, glycosyl hydrolase family 5 protein/cellulose, choline/ethanolamine kinase, and aquaporin-like protein. The aquaporin-like protein gene was evidently upregulated in dl mutant. Thus, we inferred that this gene is a potential candidate for the phenotypes. The results provide a basis to elucidate the regulatory pathway responsible for trichome formation and stamen development in tomato.