Genetic Studies of Paralyzed Mutants in Salmonella. II. Mapping of Three mot Loci by Linkage Analysis.
Genetic Studies of Paralyzed Mutants in Salmonella. II. Mapping of Three mot Loci by Linkage Analysis.
复制标题
沙门氏菌麻痹突变体的遗传学研究。
作者：
M. Enomoto
在前面的论文（ENOMOTO 1966）中报道，鼠伤寒沙门氏菌的运动基因由三个功能单位组成：motA、motB和motC。motA和B彼此相邻，但motC是分开的，并与HI基因共同转导。然而，尚未研究motA和B的染色体定位以及motC相对于H I的位置。为了分析mot位点与其它基因的连锁关系，有性重组系统是有用的。在沙门氏菌属中，没有一种Hfr菌株是从自然栖息地分离出来的。然而，许多研究是通过大肠杆菌和沙门氏菌之间的杂交进行的（BARON et al. 1959 a，B; MIYAKE and DEMEREC 1959; ZINDER 1960 a; MAKELA，LEDERBERG and LEDERBERG 1962; ~ K E L A 1964），发现获得的杂交体在进一步交配中与沙门氏菌重组（ZINDER 1960 b; DEMEREC等1960; K E L A等1962）。MAKELA（1963）、SANDERSON和DEMEREC（1965）等从感染大肠杆菌F的沙门氏菌中分离出几种Hfr突变体。coli K-12，并使沙门氏菌属物种的整体遗传分析成为可能。本文利用沙门氏菌abony Hfr突变株与沙门氏菌Fis有性重组的方法，对这三个基因座与其它染色体基因进行了连锁分析。
reported in the preceding paper (ENOMOTO 1966), motility genes of Aialmonella typhimurium consist of three functional units: motA, motB, and motC. motA and B adjoin each other but motC is separate and is transduced jointly with the HI gene. However, the chromosomal location of motA and B and the position of motC relative to H I have not been investigated. In order to analyse the linkage relationship between the mot loci and other genes, a system of sexual recombination is useful. In Salmonella species, none of the Hfr strains had been isolated from natural habitats. However, many investigations have been carried out by hybridization between Escherichia coli and Salmonella (BARON et al. 1959a, b; MIYAKE and DEMEREC 1959; ZINDER 1960a; MAKELA, LEDERBERG and LEDERBERG 1962; ~ K E L A 1964) and the hybrids obtained were found to recombine with Salmonella in further mating (ZINDER 1960b; DEMEREC et al. 1960; ~ K E L A et al. 1962). Furthermore, MAKELA (1 963), and SANDERSON and DEMEREC (1965) succeeded in isolating several Hfr mutants from Salmonella infected with F from E. coli K-12 and made possible overall genetic analysis in Salmonella species. In the present paper, the linkage analysis of the three mot loci in relation to other chromosomal genes by means of sexual recombination between Salmonella abony Hfr mutant and Salmonella Fis reported.