Tetranitromethane. A reagent for the nitration of tyrosyl residues in proteins.
Tetranitromethane. A reagent for the nitration of tyrosyl residues in proteins.
复制标题
四硝基甲烷。
DOI：
10.1021/bi00875a029
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发表时间：
1966
期刊：
影响因子：
2.9
通讯作者：
B. Vallée
中科院分区：
文献类型：
--
作者：
M. Sokolovsky;J. Riordan;B. Vallée
已发现四硝基甲烷 (TNM) 是一种特异性、温和的试剂，可在 pH 8 下硝化蛋白质的酪氨酰残基。半胱氨酸（唯一受影响的其他氨基酸）的氧化可通过在 pH 6 下进行反应来区分，其中酪氨酸不发生改变。通过色谱法，3-硝基酪氨酸已被鉴定为 TNM 与含酪氨酰的肽和蛋白质反应的产物，产生最大吸收带
Tetranitromethane (TNM) has been found to be a specific, mild reagent for the nitration of tyrosyl residues of proteins at pH 8. Oxidation of cysteine, the only other amino acidaffected, can be differentiated by carrying out the reaction at pH 6 where tyrosine is not altered. By chromatography 3-nitrotyrosine has been identified as the product of the reaction of TNM with tyrosyl-containing peptides and proteins, resulting in an absorption band with a maximum at