Enterobacter aerogenes metabolites enhance Microcystis aeruginosa biomass recovery for sustainable bioflocculant and biohydrogen production
Enterobacter aerogenes metabolites enhance Microcystis aeruginosa biomass recovery for sustainable bioflocculant and biohydrogen production
复制标题
产气肠杆菌代谢物增强铜绿微囊藻生物量回收，用于可持续生物絮凝剂和生物氢生产
DOI：
10.1016/j.scitotenv.2018.03.327
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发表时间：
2018
影响因子：
9.8
通讯作者：
徐亮
中科院分区：
文献类型：
--
作者：
徐亮
本文报道了一种利用产气肠杆菌产絮凝剂（MBF-32）对铜绿微囊藻进行生物资源回收的方法。铜绿假单胞菌作为MBF-32和生物氢可持续生产的主要底物。实验结果表明，在最佳条件下，生物絮凝效率可达90%以上。收获的M.铜绿被进一步回收作为供应必需元素的主要基质。在20 g/L湿生物量时，MBF-32的产量最高（3.6 ± 0.1 g/L）。以20 g/L葡萄糖为碳源，对铜绿假单胞菌进行发酵。最高的生物氢产量为35 mL H2/g（dw）藻类生物质，从20 g/L的M.铜绿假单胞菌与另外的10 g/L甘油。转录组分析表明MBF-32主要由多糖和酪氨酸/色氨酸蛋白组成。此外，NADH合成酶和多糖输出相关基因被发现上调。
We report a recycling bioresource involving harvesting of Microcystis aeruginosa using the bioflocculant (MBF-32) produced by Enterobacter aerogenes followed by the recovery of the harvested M. aeruginosa as themain substrate for the sustainable production of MBF-32 and biohydrogen. The experimental results indicate that the efficiency of bioflocculation exceeded 90% under optimal conditions. The harvested M. aeruginosa was further recycled as the main substrate for the supply of necessary elements. The highest yield (3.6 ± 0.1 g/L) of MBF-32 could be obtained from 20 g/L of wet biomass of M. aeruginosa with an additional 20 g/L of glucose as the extra carbon source. The highest yield of biohydrogen was 35 mL of H2/g (dw) algal biomass, obtained from.20 g/L of wet biomass of M. aeruginosa with an additional 10 g/L of glycerol. Transcriptome analyses indicated that MBF-32 was mainly composed of polysaccharide and tyrosine/tryptophan proteins. Furthermore, NADH synthase and polysaccharide export-related genes were found to be up-regulated.