Image-based tissue engineering of a total intervertebral disc implant for restoration of function to the rat lumbar spine
Image-based tissue engineering of a total intervertebral disc implant for restoration of function to the rat lumbar spine
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DOI：
10.1002/nbm.1651
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发表时间：
2012-03-01
影响因子：
2.9
通讯作者：
Bonassar, Lawrence J.
中科院分区：
文献类型：
--
作者：
Bowles, Robby D.;Gebhard, Harry H.;Bonassar, Lawrence J.
非生物全椎间盘置换术目前被用于治疗椎间盘（IVD）疾病和损伤，但这些植入物容易发生机械磨损、撕裂和可能的移位。最近，组织工程全椎间盘置换术（TE-TDR）已被研究作为一种可能的替代方案，以更充分地复制原生IVD属性。然而，TE-TDR的性能尚未在原生椎间盘空间中进行研究。在这项研究中，MRI和显微计算机断层扫描成像的大鼠脊柱被用来设计一个胶原蛋白（纤维环）/藻酸盐（髓核）TE-TDR的高度几何精度，TE-TDR和天然椎间盘尺寸之间的差异小于10%。然后将基于图像的TE-TDR植入物插入无胸腺大鼠的L4/L5椎间盘间隙（n = 10）。5)并维持16周。5只动物中有3只完全或部分维持椎间隙，蛋白聚糖和胶原组织学染色的组成与天然椎间盘相似。此外，在TE-TDR和椎体之间观察到良好的整合，以及残留的原生IVD组织。总体而言，本研究提供的证据表明，TE-TDR策略可能为患病或受伤的IVD提供临床可行的治疗。版权所有（c）2011约翰威利父子有限公司
Nonbiological total disc replacement is currently being used for the treatment of intervertebral disc (IVD) disease and injury, but these implants are prone to mechanical wear, tear and possible dislodgement. Recently, tissue-engineered total disc replacement (TE-TDR) has been investigated as a possible alternative to more fully replicate the native IVD properties. However, the performance of TE-TDRs has not been studied in the native disc space. In this study, MRI and microcomputed tomography imaging of the rat spine were used to design a collagen (annulus fibrosus)/alginate (nucleus pulposus) TE-TDR to a high degree of geometric accuracy, with less than 10% difference between TE-TDR and the native disc dimensions. Image-based TE-TDR implants were then inserted into the L4/L5 disc space of athymic rats (n?=?5) and maintained for 16 weeks. The disc space was fully or partially maintained in three of five animals and proteoglycan and collagen histology staining was similar in composition to the native disc. In addition, good integration was observed between TE-TDR and the vertebral bodies, as well as remnant native IVD tissue. Overall, this study provides evidence that TE-TDR strategies may yield a clinically viable treatment for diseased or injured IVD. Copyright (c) 2011 John Wiley & Sons, Ltd.