Dictyostelium Discoideum: A Developmental System
Dictyostelium Discoideum: A Developmental System
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盘基网柄菌：一个发育系统
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发表时间：
1976
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通讯作者：
J. Zeldis
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作者：
J. Zeldis
307号可见。过氧化物酶技术还允许对标本进行电子显微镜检查。也许有几章太多的篇幅专门介绍这项技术的个别应用，但这是诊断病毒学的一个令人兴奋的发展。病毒基因组可以在没有充分表达的情况下存在于细胞中，导致产生病毒抗原或病毒粒子，这可以通过免疫学方法检测到。因此，本书末尾有一章专门介绍与病毒核酸进行细胞杂交的技术是恰当的。这种方法虽然还处于初级阶段，但已经用于检测临床标本中的巨细胞病毒。例如，CMV基因组已经在精子的头部被证明。这本书相当强调技术，因此它的最大用处将是对板凳工人。当然，每一种技术本身都可以成为一部专著的主题。尽管如此，这本书指出了医学病毒学将很快提供快速临床有用数据的方式。盘基菌，或细胞黏菌，确实是一种令人着迷的有机体。当营养丰富时，这种有机体以阿米巴虫的形式存在，这种阿米巴虫非常满意地吞噬食物并分裂。然而，当食物供应消失时，阿米巴虫神秘地开始聚集，直到它们形成一种鼻涕虫，在黏液的外套上迁徙，直到找到一个顺从的位置。然后，鼻涕虫分化成茎和子实体，释放孢子。从每个孢子中会长出一种变形虫，重新开始整个生命过程。总结了这种生物分化过程中的大部分生物学方面。自那本书出版以来，许多工作人员开始在分子水平上研究分化过程。初步的遗传学已经建立，包括在分化过程的不同阶段有缺陷的突变体。为了研究阿米巴的生化变化，该菌适应于人工组织培养基类(无菌培养基)，以使大批阿米巴在分化过程中同步。聚集体的化学介体已被分离和表征。在过去的4年里，黏菌已经发展成为研究基因表达和分化的主要真核模型系统之一。因此，Loomis的卷是该领域人士欢迎的资源和评论。除了有一个非常完整的参考书目，文本写得非常好。由于卢米斯博士假定读者对…一无所知
307 cernible. The peroxidase technique also permits the specimen to be examined by electron microscopy. Perhaps a few too many chapters are devoted to individual applications of the technique, but it is an exciting development in diagnostic virology. Viral genomes can be present in cells without full expression leading to the production of viral antigens or virions which can be detected by immunologic methods. Hence, it is fitting that a chapter near the end of the book is devoted to the technique of cytohybridization with viral nucleic acids. This method, while in its infancy, has already been useful in the detection of cytomegalovirus in clinical specimens. For example, CMV genomes have been demonstrated in the heads of spermatozoa. This book has a rather heavy emphasis on technique; hence its greatest utility will be for the bench worker. Each technique, of course, could in itself be the subject of a monograph. Nonetheless, the volume points to the way in which medical virology will soon provide rapid clinically useful data. Dictyostelium discoideum, or the cellular slime mold, is truly a fascinating organism. When there are nutrients in abundance, the organism exists as amoebae that quite contentedly phagocytize food and divide. However, when the food supply disappears the amoebae mysteriously begin to aggregate until they form a slug that migrates on a coat of slime until it finds an amenable location. The slug then differentiates into a stalk with a fruiting body that releases spores. From each spore will emerge an amoeba to start the whole life process over again. summarized most of the biological aspects of the differentiation process of this organism. Since that book was published, many workers have begun to study the differentiation process at a molecular level. Rudimentary genetics were established that included mutants defective in various stages of the differentiation process. The organism was adapted to artificial tissue culturelike media (axenic media) so that large batches of amoebae could be synchronized during differentiation in order to study biochemical changes. Chemical mediators of aggregation have been isolated and characterized. In the past 4 years, the slime mold has evolved into one of the major eukaryotic model systems to study gene expression and differentiation. Hence, Loomis' volume is a welcome resource and review for people in the field. Besides having a very complete bibliography, the text is very well written. Since Dr. Loomis assumes no knowledge by the reader concerning …