Solid-State NMR for Bacterial Biofilms.
Solid-State NMR for Bacterial Biofilms.
复制标题
细菌生物膜的固态核磁共振。
DOI：
10.1080/00268976.2013.837983
复制
发表时间：
2014
影响因子：
1.7
通讯作者：
Cegelski,Lynette
中科院分区：
文献类型：
--
作者：
Reichhardt,Courtney;Cegelski,Lynette
细菌通过精心设计细胞外基质来包裹细胞，从而与表面和彼此联系在一起，创造出被称为生物膜的群落。生物膜在某些生态环境中是有益的，但也有助于严重和慢性传染病的发病。需要新的方法和定量测量来定义细菌生物膜的组成和结构，以帮助推动干扰生物膜组装的策略的开发。固体核磁共振技术特别适合于研究难溶和复杂的大分子和全细胞系统。本文重点介绍了三个实施固态核磁共振的例子，以提供对细菌生物膜组成的见解，以及当细胞过渡到生物膜生活方式时细胞壁组成的变化。最近，固态核磁共振测量提供了对大肠杆菌大肠杆菌生物膜细胞外基质中蛋白质和多糖成分的全面统计，并将我们对基质组成的定性描述转化为允许在样品之间进行定量比较的化学参数。我们提供了整个生物膜样本(细胞和细胞外基质)的额外数据，以补充仅限基质的分析。利用固体核磁共振技术研究细菌生物膜是一个充满机遇的激动人心的研究领域，本文最后阐述了这一领域的一些突出问题和未来发展方向。
Bacteria associate with surfaces and one another by elaborating an extracellular matrix to encapsulate cells, creating communities termed biofilms. Biofilms are beneficial in some ecological niches, but also contribute to the pathogenesis of serious and chronic infectious diseases. New approaches and quantitative measurements are needed to define the composition and architecture of bacterial biofilms to help drive the development of strategies to interfere with biofilm assembly. Solid-state nuclear magnetic resonance (NMR) is uniquely suited to the examination of insoluble and complex macromolecular and whole-cell systems. This article highlights three examples that implement solid-state NMR to deliver insights into bacterial biofilm composition and changes in cell-wall composition as cells transition to the biofilm lifestyle. Most recently, solid-state NMR measurements provided a total accounting of the protein and polysaccharide components in the extracellular matrix of anEscherichia colibiofilm and transformed our qualitative descriptions of matrix composition into chemical parameters that permit quantitative comparisons among samples. We present additional data for whole biofilm samples (cells plus the extracellular matrix) that complement matrix-only analyses. The study of bacterial biofilms by solid-state NMR is an exciting avenue ripe with many opportunities and we close the article by articulating some outstanding questions and future directions in this area.