Bacteria in midguts of field-collected Anopheles albimanus block Plasmodium vivax sporogonic development
Bacteria in midguts of field-collected Anopheles albimanus block Plasmodium vivax sporogonic development
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DOI：
10.1603/0022-2585-40.3.371
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发表时间：
2003-05-01
影响因子：
2.1
通讯作者：
Hernandez-Avila, JE
中科院分区：
文献类型：
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作者：
Gonzalez-Ceron, L;Santillan, F;Hernandez-Avila, JE
调查了来自墨西哥南部的白纹伊蚊和野外采集的白纹伊蚊的中肠细菌感染情况。粘质沙雷氏菌、阴沟肠杆菌和羊肠杆菌2、肠杆菌属和沙雷氏菌属。从1998年采集的现场样品中分离到2株，但仅检出肠杆菌属(Enterbacter sp.)在1997年的田间样品中被发现，从昆虫标本中没有分离到细菌。这些细菌与间日疟原虫感染的血液一起被提供给无菌杀虫剂AN。7d后检测感染蚊虫的数量和卵囊密度。间日疟原虫感染与EN混合感染的蚊子。Amnigus，2 en.阴沟肠杆菌和S的卵囊密度分别为对照蚊虫的53倍、17倍和210倍，混合感染EN蚊虫的卵囊平均密度降低。泄殖腔比对照组低2.5倍。与对照组相比，感染了大斑潜蝇的蚊子的死亡率高出13倍。蚊子现场种群的整体中肠细菌感染可能会影响间日疟原虫的传播，并可能有助于解释该地区观察到的疟疾发病率的年度变化。
Bacterial infections were investigated in midguts of insectary and field-collected Anopheles albimanusWeidemann from southern Mexico. Serratia marcescens, Enterobacter cloacae and Enterobacter amnigenus 2, Enterobacter sp., and Serratia sp. were isolated in field samples obtained in 1998, but only Enterobacter sp. was recovered in field samples of 1997 and no bacteria were isolated from insectary specimens. These bacteria were offered along with Plasmodium vivax infected blood to aseptic insectary An. albimanus, and the number of infected mosquitoes as well as the oocyst densities assessed after 7d. Plasmodium vivax infections in mosquitoes co-infected with En. amnigenus, 2 En. cloacae, and S, marcensces were 53, 17, and 210 times, respectively, lower than in control mosquitoes, and the mean oocyst density in mosquitoes co-infected with En. cloacae was 2.5 times lower than in controls. Mortality was 13 times higher in S. marcensces-infected mosquitoes compared with controls. The overall midgut bacteria] infection in mosquito field populations may influence P. vivax transmission, and could contribute to explain the annual variations in malaria incidence observed in the area.