Wound Healing Effect of Kaempferol in Diabetic and Nondiabetic Rats
Wound Healing Effect of Kaempferol in Diabetic and Nondiabetic Rats
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DOI：
10.1016/j.jss.2018.08.009
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发表时间：
2019-01-01
影响因子：
2.2
通讯作者：
Darcan, Sinan
中科院分区：
文献类型：
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作者：
Ozay, Yusuf;Guzel, Sevda;Darcan, Sinan
背景：以前有人认为黄酮类化合物在伤口愈合中起作用。然而，迄今为止，有限的信息是可用于伤口愈合效果的山奈酚（KM），这属于类黄酮。本研究的目的是确定KM的伤口愈合效果。材料和方法：KM与两种不同浓度（0.5%和1% [重量/重量，w/w]）的伤口愈合效果进行了评估，在切口和切除伤口模型糖尿病和非糖尿病大鼠宏观，生物力学，生化和组织病理学分析。链脲佐菌素诱发糖尿病。KM软膏采用硬脂酸乙二醇酯：丙二醇：液体石蜡（3：6：1）的混合物制备; 0.5g软膏局部涂抹于创面，每天一次，持续7和14 d。在第0、7和14天，对伤口拍照，并对伤口进行肉眼检查。7和14 d后，羟脯氨酸水平，生物力学分析和组织病理学参数评估（上皮再生、肉芽组织厚度、血管生成、炎症的存在、胶原沉积、纤维化的存在、真皮炎症的程度和肥大细胞的数量）。糖尿病切除创面和非糖尿病切口创面愈合效果最好1%（w/w）KM软膏处理14 d后，肉眼检查，两组的感染率分别为92.12%和94.17%。用1%（w/w）KM软膏治疗的非糖尿病切除（第14天）和切口（第7天）伤口显示出统计学上高于对照组的羟脯氨酸水平（分别为2.84和2.07 μ g/mg，P < 0.01）。糖尿病组和非糖尿病组切创术后第7和14天的再上皮化评分（P < 0.05和P < 0.01）和切创术后第14天的再上皮化评分（P < 0.05）均显著高于对照组。0.5%（w/w）KM软膏处理后第14天，非糖尿病组和糖尿病组的抗张强度分别为0.92和0.82 g/s。结论：KM是一种治疗非糖尿病和糖尿病创面的有效外用创面愈合剂。(C)2018爱思唯尔公司All rights reserved.
Background: Flavonoids have previously been suggested to play a role in wound healing. To date, however, limited information is available on the wound healing effect of kaempferol (KM), which belongs to the class of flavonoids. The objective of this study was to determine the wound healing effects of KM.Materials and methods: The wound healing effects of KM with two different concentrations (0.5% and 1% [weight/weight, w/w]) were evaluated in incisional and excisional wound models on diabetic and nondiabetic rats by macroscopic, biomechanical, biochemical, and histopathological analyses. Diabetes was induced by streptozotocin. The KM ointments were prepared using a mixture of glycol stearate:propylene glycol:liquid paraffin (3:6:1); 0.5 g of the ointments were topically applied on the wounded areas once a day for 7 and 14 d. On days 0, 7, and 14, wounds were photographed, and macroscopic examination of the wounds was performed. After 7 and 14 d, hydroxyproline levels, biomechanical analysis, and histopathological parameters (reepithelialization, thickness of granulation tissue, angiogenesis, presence of inflammation, deposition of collagen, presence of fibrosis, degree of dermal inflammation, and number of mast cells) were assessed.Results: The best wound healing effect was observed in the diabetic excisional and nondiabetic incisional wounds (92.12% and 94.17%, respectively) treated with 1% (w/w) KM ointment for 14 d according to macroscopic examination. The nondiabetic excisional (14th day) and incisional (7th day) wounds treated with 1% (w/w) KM ointment showed statistically higher levels of hydroxyproline than the control groups (2.84 and 2.07 mu g/mg, respectively, P < 0.01). Reepithelialization scores of KM-treated diabetic and nondiabetic excisional wounds on both 7 and 14 d (P < 0.05 and P < 0.01) and incisional wounds on the day 14 (P < 0.05) were significantly higher than controls. The maximum tensile strength was observed in nondiabetic and diabetic groups (0.92 and 0.82 g/s, respectively) treated with 0.5% (w/w) KM ointment on day 14.Conclusions: Thus, KM appears to be an effective topical wound healing agent in the treatment of both nondiabetic and diabetic wounds. (C) 2018 Elsevier Inc. All rights reserved.