Synthesis of collagen-like peptide polymers by native chemical ligation
Synthesis of collagen-like peptide polymers by native chemical ligation
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DOI：
10.1021/ma0514065
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发表时间：
2005-09-06
期刊：
影响因子：
5.5
通讯作者：
Hartgerink, JD
中科院分区：
文献类型：
--
作者：
Paramonov, SE;Gauba, V;Hartgerink, JD
本文报道了采用固相法、聚合和自组装相结合的方法合成高相对分子质量的类胶原蛋白聚合物。最终产品是一种纳米纤维网状物，它保持了胶原蛋白三螺旋的特征圆二色信号，纳米纤维直径为10-20 nm，类似于天然胶原原纤维。该方法利用N-端半胱氨酸和C-端硫酸酯来实现多肽的选择性头尾聚合，而不需要保护基团，并且在中性的水条件下多肽可以采用折叠构象。用尺寸排斥层析、圆二色谱和透射电子显微镜对所合成的多肽聚合物进行了表征。
We report on the synthesis of high molecular weight collagen-like peptide polymers prepared by a combination of solid phase peptide synthesis, polymerization, and self-assembly. The final product is a mesh of nanofibers that maintains the characteristic circular dichroism signal for collagen triple helices and the nanofiber diameter is 10-20 nm, similar to natural collagen fibrils. This method utilizes an N-terminal cysteine and C-terminal thioester to achieve selective head to tail polymerization of peptides without the need for protecting groups and under neutral aqueous conditions in which the peptide may adopt a folded conformation. The synthesized peptide polymers were characterized by size-exclusion chromatography, circular dichroism spectroscopy, and transmission electron microscopy.