Quantitatively resolving multivalent interactions on a macroscopic scale using force spectroscopy.
Quantitatively resolving multivalent interactions on a macroscopic scale using force spectroscopy.
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DOI：
10.1039/c5cc10535h
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发表时间：
2016-03-04
期刊：
影响因子：
--
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Hu Q;Yang H;Wang Y;Xu S
多价相互作用仍然难以表征并因此难以控制，特别是在宏观尺度上。使用力诱导剩余磁化光谱 (FIRMS)，我们解析了毫米级表面上的单生物素、双生物素和三生物素-链霉亲和素相互作用、多价 DNA 相互作用和 CXCL12-CXCR4 相互作用。我们的结果表明 FIRMS 是一种系统解决和控制多价相互作用形成的可行方法。
Multivalent interactions remain difficult to be characterized and consequently controlled, particularly on a macroscopic scale. Using force-induced remnant magnetization spectroscopy (FIRMS), we have resolved the single-, double-, and triple- biotin—streptavidin interactions, multivalent DNA interactions and CXCL12-CXCR4 interactions, on millimetre-scale surfaces. Our results establish FIRMS as a viable method for systematic resolution and controlled formation of multivalent interactions.