Influence of ovarian hormones on prolactin release in the rhesus monkey.
Influence of ovarian hormones on prolactin release in the rhesus monkey.
复制标题
卵巢激素对恒河猴催乳素释放的影响。
作者：
J. Milmore
用两种不同的方法采集雌性恒河猴的血样，用放射免疫法测定血清催乳素水平。氯胺酮麻醉猴子的催乳素水平差异很大，未麻醉猴子的催乳素水平（23.5±4.2 ng/ ml. mean±SEM）与去卵巢猴子的催乳素水平（24.0±5.1 ng/ ml）无明显差异。相比之下，当从灵长类动物约束椅上未麻醉的猴子身上提取血液样本时。催乳素8.2±1.7 ng！在未切除卵巢的猴子中降至2.5±0.4 ng/ Ml。在去卵巢的猴子中，在注射促甲状腺激素释放激素（TRU）后，泌乳素的峰值释放。24 ug。Iv .)或匹莫齐（0.2 mg/kg Iv .）分别减少72%和68%。分别。替代雌二醇- 17。通过硅橡胶胶囊植入1-6个月，恢复去卵巢猴子对TRH的反应。7只去卵巢猕猴经雌激素治疗2-5个月后，垂体重量为81.0±3.1 mg，而5只去卵巢猕猴的垂体重量为61.1±2.3 mg （P< 0.01）。雌激素使垂体催乳素浓度从452±76 ng/mg增加到974±lOS ng/mg （P< 0.05）。O我)。本研究结果表明，在适当的实验条件下，卵巢切除可减少催乳素的释放。此外。雌激素似乎参与了这一作用。因为雌激素替代恢复了trh诱导的催乳素释放，增加了去卵巢猴子的垂体重量和催乳素浓度。
Blood samples were collected from female rhesus monkeys by two different techniques and serum prolactin levels were determined by radioimmunoassay. Prolactin levels in samples from ketamine anesthetized monkeys varied widely and there was no apparent difference between those of intact (23.5 ±4.2 ng/ ml. mean ± SEM) and ovariectomized (24.0±5.1 ng/ ml) monkeys. In contrast, when blood samples were taken from unanesthetized monkeys in primate restraint chairs. prolactin levels were 8.2±1.7 ng!ml in intact monkeys and were reduced to 2.5 ±0.4 ng/ml in ovariectomized monkeys. In ovariectomized monkeys, the peak release of prolactin following injection of either thyrotropin releasing hormone (TRU. 24 ug. iv.) or pimozide (0.2 mg/kg iv.) were reduced by 72% and 68%. respectively. Replacement of estradiol-l7�. by means of Silastic capsule implants for periods of 1-6 months, restored the responsiveness of ovariectomized monkeys to TRH. Pituitary weights in 7 ovariectomized monkeys treated with estrogen for 2-5 months were 81.0±3.1 mg as compared to 61.1±2.3 mg for 5 ovariectomized controls (P<O.OOl). Estrogen treatment also increased pituitary prolactin concentrations from 452± 76 ng/mg so 974± lOS ng/mg (P<0.O I). The results of this study demonstrate that ovariecsomy diminishes the release of prolactin under suitable experimental conditions. Furthermore. is appears that estrogens are involved in this effect. since estrogen replacement restores she TRH-induced release of prolactin and increases bosh pituitary weight and prolactin concentration of ovariectomized monkeys.