Hosts and pathogen detection for immature stages of Ixodes ricinus (Acari: Ixodidae) in North-Central Spain
Hosts and pathogen detection for immature stages of Ixodes ricinus (Acari: Ixodidae) in North-Central Spain
复制标题
DOI：
10.1007/s10493-005-3271-6
复制
发表时间：
2005-12-01
影响因子：
2.2
通讯作者：
Gray, JS
中科院分区：
文献类型：
--
作者：
Estrada-Peña, A;Osácar, JJ;Gray, JS
为了确定南部种群未成熟阶段的寄主，我们于1998年和1999年5-6月和8-9月在西班牙中北部地区诱捕了啮齿动物和鸟类。诱捕率最高的是黑线姬鼠(230只)和灰仓鼠(99只)，幼虫感染率春季分别为49%和81%，夏季分别为21%和41%。幼虫在春季的平均寄生量为19.8只，夏季平均为3.4只，均高于黑腹小蜂(6.1只和1.2只)。没有从啮齿动物身上采集到若虫。幼虫在不同鼠种间的分布格局有较大差异，但不同季节间差异不大。最常见的诱捕鸟类是蝶科成员，春季幼虫和若虫的感染率分别为70%和10%，夏季为20%-15%。用分子生物学方法对2002年6月和2003年6月在同一地点采集的若虫幼虫寄主和寄生病原菌进行了鉴定。研究表明，大多数扁虱以鸟类为食，少数以啮齿动物和反刍动物为食。在这些硬蜱中检测到的病原体包括微小巴贝斯虫、加里氏疏螺旋体、瓦氏巴氏杆菌和弗氏巴氏杆菌。在一只显然以野猪为食的扁虱体内检测到了阿氏疏螺旋体。
To determine hosts of the immature stages of a southern population of Ixodes ricinus, we trapped rodents and birds in an area of north-central Spain in May-June and August-September of 1998 and 1999. The most frequently trapped rodents were Apodemus sylvaticus (230 specimens) and Clethrionomys glareolus (99), with a larval infestation prevalence of 49% and 81%, respectively (in spring) and 21% and 41% (in summer). C. glareolus was always more heavily parasitized by larvae (mean numbers 19.8 in spring, 3.4 in summer) than A. sylvaticus (6.1 and 1.2, respectively). No nymphs were collected from the rodents. The larval distribution pattern differed considerably between rodent host species but not according to the season of the year. The most commonly trapped birds were members of the Turdidae, which had infestation incidences of about 70% (larvae) and 10% (nymphs) in spring and 20-15% in summer. We also used molecular methods for the identification of reservoir hosts of the larval ticks and of the pathogens they contained, from nymphs collected in the same site in June of 2002 and 2003. The study showed that most of the ticks had fed on birds and few on rodents and ruminants. Pathogens detected in these ticks included Babesia microti, Borrelia garinii, B. valaisiana and B. afzelii. Borrelia afzelii was detected in a tick that had apparently fed on a wild boar.