Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals
Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals
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DOI：
10.1073/pnas.97.13.7090
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发表时间：
2000-06-20
影响因子：
11.1
通讯作者：
Landick, R
中科院分区：
文献类型：
--
作者：
Artsimovitch, I;Landick, R
RNA 聚合酶导致的转录本延伸是不连续的，并且会被发挥关键调节作用的暂停所打断。我们在这里展示了两种不同类别的暂停信号会破坏伸长。在肠道细菌中发现的 I 类暂停依赖于新生 RNA 结构与 RNA 聚合酶的相互作用，将 3' OH 移离催化中心。 II 类暂停可能在真核生物中占主导地位，导致 RNA 聚合酶沿着 DNA 和 RNA 向后滑动，并用新生 RNA 封闭活性位点。这些暂停对反义寡核苷酸的反应不同。焦磷酸盐。 GreA，以及一般伸长因子 NusA 和 NusG。相反，RNA 聚合酶的替换会增加或降低 RNA 合成速率，对两种暂停类别的影响类似。我们认为，这两种暂停类别以及停滞和终止均源自其自身与 NTP 底物的弱结合的共同中间体。
Transcript elongation by RNA polymerase is discontinuous and interrupted by pauses that play key regulatory roles. We show here that two different classes of pause signals punctuate elongation. Class I pauses, discovered in enteric bacteria, depend on interaction of a nascent RNA structure with RNA polymerase to displace the 3' OH away from the catalytic center. Class II pauses, which may predominate in eukaryotes, cause RNA polymerase to slide backwards along DNA and RNA and to occlude the active site with nascent RNA. These pauses differ in their responses to antisense oligonucleotides. pyrophosphate. GreA, and general elongation factors NusA and NusG. In contrast, substitutions in RNA polymerase that increase or decrease the rate of RNA synthesis affect both pause classes similarly. We propose that both pause classes, as well as arrest and termination, arise from a common intermediate that itself binds NTP substrate weakly.