Inhibition of Lipopolysaccharide (LPS)-Induced Inflammatory Responses by Sargassum hemiphyllum Sulfated Polysaccharide Extract in RAW 264.7 Macrophage Cells
Inhibition of Lipopolysaccharide (LPS)-Induced Inflammatory Responses by Sargassum hemiphyllum Sulfated Polysaccharide Extract in RAW 264.7 Macrophage Cells
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DOI：
10.1021/jf1043647
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发表时间：
2011-03-09
影响因子：
6.1
通讯作者：
Wu, Chang-Jer
中科院分区：
文献类型：
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作者：
Hwang, Pai-An;Chien, Shih-Yung;Wu, Chang-Jer
半叶马尾藻是一种常见于东亚沿着的褐藻，长期以来一直是一种传统中药。S.半叶藓已显示出抗炎作用;然而，其机制尚未被清楚地阐明。本研究探讨了S.疝的生物医学效应。S.首先制备半叶藻硫酸多糖提取物（SHSP），以脂多糖（LPS）激活的小鼠巨噬细胞系（RAW 264.7）为模型系统。促炎性细胞因子（包括IL-1 β、IL-6、TNF-α和NO）的分泌特征被发现在1-5 mg/mL剂量范围的SHSP治疗中显著降低。RT-PCR结果显示SHSP可抑制LPS诱导的IL-β、NOS和考克斯-2 mRNA表达，且呈剂量依赖性。在蛋白质水平上，Western印迹分析证实了细胞质/细胞核中NF-κ B（p65）的类似结果。因此，SHSP的抗炎作用可能与下调NF-κ B B表达有关。
Sargassum hemiphylium, a kind of brown seaweed generally found along coastlines in East Asia, has long served as a traditional Chinese medicine. S. hemiphyllum has shown an anti-inflammatory effect; however, its mechanism has not been elucidated dearly. This study explored S. herniphyllum for its biomedical effects. S. hemiphyllum sulfated polysaccharide extract (SHSP) was first prepared; the mouse macrophage cell line (RAW 264.7) activated by lipopolysaccharide (LPS) was used as a model system. The secretion profiles of pro-inflammatory cytokines, including IL-1 beta, IL-6, TNF-alpha, and NO, were found significantly to be reduced in 1-5 mg/mL dose ranges of SHSP treatments. RT-PCR analysis suggested SHSP inhibits the LPS-induced mRNA expressions of IL-beta, NOS, and COX-2 in a dose-dependent manner. At protein levels, Western blot analysis demonstrated a similar result for NF-kappa B (p65) in cytosol/nuclear. Taken together, the anti-inflammatory properties of SHSP may be attributed to the down-regulation of NF-kappa B in nucleus.