Association between genotypic diversity and biofilm production in group B Streptococcus.
Association between genotypic diversity and biofilm production in group B Streptococcus.
复制标题
DOI：
10.1186/s12866-016-0704-9
复制
发表时间：
2016-05-20
期刊：
影响因子：
4.2
通讯作者：
Manning SD
中科院分区：
文献类型：
--
作者：
Parker RE;Laut C;Gaddy JA;Zadoks RN;Davies HD;Manning SD
B族链球菌（GBS）是败血症和脑膜炎的主要原因，也是早产和死胎的重要因素。生物被膜的产生被认为是重要的GBS发病机制与许多其他因素，包括系统发育谱系和毒力因子，如皮利和胶囊类型。然而，对这些组成部分的汇合点缺乏全面的了解。为了鉴定生物膜表型、菌毛特征和谱系之间的关联，使用体外测定评估了来自无症状携带者、侵袭性疾病病例和牛乳腺炎病例的293株菌株的生物膜产生。多位点序列类型（ST）配置文件，菌毛岛配置文件，和分离源与生物膜的生产。来自侵袭性疾病病例和/或属于ST-17和ST-19谱系的菌株更有可能形成弱生物膜，而产生强生物膜的菌株更频繁地从无症状定植的个体中回收。这些数据表明，生物膜的产生是GBS的谱系特异性性状，并可能促进代表ST 17和19以外谱系的菌株的定殖。本文的发现还表明，在治疗孕妇时必须考虑生物膜，特别是对于具有重GBS定殖的妇女。本文的在线版本（doi：10.1186/s12866-016-0704-9）包含补充材料，可供授权用户使用。
Group B Streptococcus (GBS) is a leading cause of sepsis and meningitis and an important factor in premature and stillbirths. Biofilm production has been suggested to be important for GBS pathogenesis alongside many other elements, including phylogenetic lineage and virulence factors, such as pili and capsule type. A complete understanding of the confluence of these components, however, is lacking. To identify associations between biofilm phenotype, pilus profile and lineage, 293 strains from asymptomatic carriers, invasive disease cases, and bovine mastitis cases, were assessed for biofilm production using an in vitro assay. Multilocus sequence type (ST) profile, pilus island profile, and isolate source were associated with biofilm production. Strains from invasive disease cases and/or belonging to the ST-17 and ST-19 lineages were significantly more likely to form weak biofilms, whereas strains producing strong biofilms were recovered more frequently from individuals with asymptomatic colonization. These data suggest that biofilm production is a lineage-specific trait in GBS and may promote colonization of strains representing lineages other than STs 17 and 19. The findings herein also demonstrate that biofilms must be considered in the treatment of pregnant women, particularly for women with heavy GBS colonization. The online version of this article (doi:10.1186/s12866-016-0704-9) contains supplementary material, which is available to authorized users.