Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
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DOI：
10.3390/metabo9030039
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发表时间：
2019-02
期刊：
影响因子：
4.1
通讯作者：
D. White;Paul A Rooks;Susan A. Kimmance;K. Tait;Mark Jones;G. Tarran;Charlotte Cook;C. Llewellyn
中科院分区：
文献类型：
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作者：
D. White;Paul A Rooks;Susan A. Kimmance;K. Tait;Mark Jones;G. Tarran;Charlotte Cook;C. Llewellyn
我们评估营养限制对小球藻极性脂质类/种细胞组成的影响。使用现代极性脂质组学分析方法（液相色谱-串联质谱; LC-MS/MS）。总极性脂质浓度最高的营养丰富（HN）的文化与单半乳糖二酰甘油（MGDG），磷脂酰甘油（PG），磷脂酰胆碱（PC），磷脂酰乙醇胺（PE）类浓度显着减少营养耗尽（LN）的文化。此外，MGDG相对于总极性脂质的丰度减少，而双半乳糖基二酰甘油（DGDG）的相对丰度增加，记录在LN文化。在HN培养物中，极性脂质物质组成在整个培养过程中保持相对恒定，具有与酰基部分相关的高度不饱和度。相反，在LN文化脂质种类的组合物转向更大的饱和度的酰基部分。多变量分析显示，许多物种丰度的变化导致LN和HN培养物之间的差异，但某些物种的影响占主导地位，例如，MGDG减少34：7（18：3/16：4）。结果表明，小球藻。显着改变其极性脂质体在响应营养限制，这是讨论的生理意义和极性脂质生产应用微藻生产系统。
We evaluate the effects of nutrient limitation on cellular composition of polar lipid classes/species in Chlorella sp. using modern polar lipidomic profiling methods (liquid chromatography–tandem mass spectrometry; LC-MS/MS). Total polar lipid concentration was highest in nutrient-replete (HN) cultures with a significant reduction in monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), phosphatidylcholine (PC), and phosphatidylethanolamine (PE) class concentrations for nutrient-deplete (LN) cultures. Moreover, reductions in the abundance of MGDG relative to total polar lipids versus an increase in the relative abundance of digalactosyldiacylglycerol (DGDG) were recorded in LN cultures. In HN cultures, polar lipid species composition remained relatively constant throughout culture with high degrees of unsaturation associated with acyl moieties. Conversely, in LN cultures lipid species composition shifted towards greater saturation of acyl moieties. Multivariate analyses revealed that changes in the abundance of a number of species contributed to the dissimilarity between LN and HN cultures but with dominant effects from certain species, e.g., reduction in MGDG 34:7 (18:3/16:4). Results demonstrate that Chlorella sp. significantly alters its polar lipidome in response to nutrient limitation, and this is discussed in terms of physiological significance and polar lipids production for applied microalgal production systems.