Recombinants between Deformed wing virus and Varroa destructor virus-1 may prevail in Varroa destructor-infested honeybee colonies
Recombinants between Deformed wing virus and Varroa destructor virus-1 may prevail in Varroa destructor-infested honeybee colonies
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DOI：
10.1099/vir.0.025965-0
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发表时间：
2011-01-01
影响因子：
3.8
通讯作者：
Ryabov, Eugene V.
中科院分区：
文献类型：
--
作者：
Moore, Jonathan;Jironkin, Aleksey;Ryabov, Eugene V.
我们已经使用高通量Illumina测序来鉴定变形翅病毒（DWV）和狄斯瓦螨病毒-1（VDV-1）之间的新型重组体，其在蜜蜂和狄斯瓦螨中积累到比DWV更高的水平。重组体VDV-1（VVD）和VDV-1（DVD 1）在5 '-UTR和编码结构（衣壳）和非结构病毒蛋白的区域之间表现出交叉。这意味着基因组是模块化的，每个区域可以独立进化，如人类肠道病毒所示。用观察到的重组体和DWV的混合物感染个体蜜蜂蛹。在蜜蜂蛹和相关螨中观察到VDV-1（DVD）水平之间的强相关性，表明这种具有DWV衍生的5 '-UTR和侧接VDV-1衍生的衣壳蛋白编码区的非结构蛋白区的重组体比亲本DWV或携带VDV-1衍生的5'-UTR的重组体（VDV-1（VVD））更适合于在狄斯韦托弧菌和蜜蜂之间传播。
We have used high-throughput Illumina sequencing to identify novel recombinants between Deformed wing virus (DWV) and Varroa destructor virus-1 (VDV-1), which accumulate to higher levels than DWV in both honeybees and Varroa destructor mites. The recombinants, VDV-1(VVD) and VDV-1(DVD1) exhibit crossovers between the 5'-UTR and the regions encoding the structural (capsid) and non-structural viral proteins. This implies that the genomes are modular and that each region may evolve independently, as demonstrated in human enteroviruses. Individual honeybee pupae were infected with a mixture of observed recombinants and DWV. A strong correlation was observed between VDV-1(DVD) levels in honeybee pupae and associated mites, suggesting that this recombinant, with a DWV-derived 5'-UTR and non-structural protein region flanking a VDV-1-derived capsid-encoding region, is better adapted to transmission between V. destructor and honeybees than the parental DWV or a recombinant bearing the VDV-1-derived 5'-UTR (VDV-1(VVD)).