Single GST-P positive liver cells--putative initiated hepatocytes.
Single GST-P positive liver cells--putative initiated hepatocytes.
复制标题
单个 GST-P 阳性肝细胞——推定的起始肝细胞。
作者：
M. Moore;K. Nakagawa;K. Satoh;T. Ishikawa;Kiyomi Sato
肝组织的免疫组织化学研究48小时后，单剂量的肝癌的二乙基亚硝胺（DEN），二甲基亚硝胺，黄曲霉毒素B1和甲基偶氮甲醇乙酸酯揭示了一个单一的谷胱甘肽S-转移酶胎盘型（GST-P）阳性肝细胞的人口的产生。产率与致癌物剂量有关。虽然混合功能酶诱导剂苯巴比妥钠（PB），甲基胆蒽（Mech），多氯联苯（PCB）和异黄樟油素（IS）本身并不诱导可比的单细胞病变，它们的应用之前，DEN引起显着改变，所得的GST-P阳性肝细胞观察到的数量。而PB和IS与产量下降，Mech和PCB，相反，带来了增加。3-氨基苯甲酰胺管理和部分肝切除术的影响，在致癌剂治疗后的不同时间进行调查的结果也指出了一个“启动”的字符的病变，并建议GST-P可能是一个有用的标志物，用于分析相关因素的肝癌发生的启动阶段。因此，致癌物代谢，DNA加合物的形成和修复，毒性和增殖之间的相互作用可以评估的酶改变的孤立肝细胞的数量。
Immunohistochemical investigation of liver tissue 48 h after single doses of the hepatocarcinogens diethylnitrosamine (DEN), dimethylnitrosamine, aflatoxin B1 and methylazoxymethanol acetate revealed the generation of a population of single glutathione S-transferase placental form (GST-P) positive hepatocytes. Yield was carcinogen dose dependent. Although the mixed function enzyme inducers sodium phenobarbital (PB), methylcholanthrene (Mech), polychlorinated biphenyls (PCB) and isosafrole (IS) did not in themselves induce comparable single cell lesions, their application prior to DEN caused significant alteration in the resultant numbers of GST-P positive hepatocytes observed. While PB and IS were associated with decreased yield, Mech and PCB, in contrast, brought about an increase. The results gained from investigation of the effects of 3-aminobenzamide administration and partial hepatectomy carried out at different times after carcinogen treatment also pointed to an 'initiated' character for the lesions and suggest that GST-P may be a useful marker for analyzing factors relevant to the initiation stage of hepatocarcinogenesis. Thus the interplay between carcinogen metabolism, DNA adduct formation and repair, toxicity and proliferation may be assessable in terms of numbers of enzyme-altered solitary hepatocytes.